Ribonuclease A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-648
![Western Blot: Ribonuclease A Antibody [NB600-648]](https://resources.bio-techne.com/images/products/Ribonuclease-A-Antibody-Western-Blot-NB600-648-img0004.jpg "Western Blot: Ribonuclease A Antibody [NB600-648]")
Key Product Details
Species Reactivity
Bovine
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Ribonuclease A [Bovine pancreas] (Uniprot: P61823)
Reactivity Notes
Cross reactivity against Ribonuclease A from other tissues and species may occur but have not been specifically determined
Localization
Secreted
Specificity
Cross reactivity against Ribonuclease A from other tissues and species may occur but have not been specifically determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine pancreas]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Ribonuclease A Antibody
Western Blot: Ribonuclease A Antibody [NB600-648]
Western Blot: Ribonuclease A Antibody [NB600-648] - Analysis using Biotin conjugate of NB600-648. Lane 1: Ribonuclease A (Bovine Pancreas). Load: 50 ng per lane. Primary antibody: Rabbit anti-Ribonuclease A Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 16.5 kDa, 16.5 kDa for Ribonuclease A. Other band(s): Possible dimerization.![Western Blot: Ribonuclease A Antibody [NB600-648]](https://resources.bio-techne.com/images/products/Ribonuclease-A-Antibody-Western-Blot-NB600-648-img0002.jpg "Western Blot: Ribonuclease A Antibody [NB600-648]")
Western Blot: Ribonuclease A Antibody [NB600-648]
Western Blot: Ribonuclease A Antibody [NB600-648] - Lane 1: Ribonuclease A (Bovine Pancreas). Load: 50 ng per lane. Primary antibody: Ribonuclease A (Bovine Pancreas) Antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 16.5 kDa, 16.5 kDa for Ribonuclease A.Applications for Ribonuclease A Antibody
Application
Recommended Usage
ELISA
1:5000-1:20000
Immunoprecipitation
1:100
Western Blot
1:500-1:5000
Application Notes
This product has been tested by western blot and is suitable to be assayed against 1.0 ug of Ribonuclease A [Bovine pancreas] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:180,000 is suggested for this product.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Ribonuclease A
Alternate Names
EC 3.1.27.5, HP-RNase, MGC12408, RIB1, RIB-1, Ribonuclease 1, Ribonuclease A, ribonuclease pancreatic, ribonuclease, RNase A family, 1 (pancreatic), RNase A, RNase UpI-1, RNS1RNase 1
Gene Symbol
RNASE1
UniProt
Additional Ribonuclease A Products
Product Documents for Ribonuclease A Antibody
Product Specific Notices for Ribonuclease A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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