Ryanodine Receptor 2 Antibody (C3-33) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80143

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Amphibian, Bovine, Canine, Chicken, Fish, Guinea Pig, Rabbit

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # C3-33

Format

BSA Free

Concentration

1 mg/ml

View all Ryanodine Receptor 2 Antibodies »

Product Specifications

Immunogen

Canine cardiac ryanodine receptor (RyR2)

Localization

Membrane; Multi-pass membrane protein

Specificity

Detects Ryanodine Receptor 2. Also shows cross-reactivity with Ryanodine Receptor 1 in amphibian, canine, chicken, fish, guinea pig and rat tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Ryanodine Receptor 2 Antibody (C3-33) - BSA Free

Immunocytochemistry/ Immunofluorescence: Ryanodine Receptor 2 Antibody (C3-33) - BSA Free [NBP2-80143]

Immunocytochemistry/ Immunofluorescence: Ryanodine Receptor 2 Antibody (C3-33) - BSA Free [NBP2-80143]

Immunocytochemistry/Immunofluorescence: Ryanodine Receptor 2 Antibody (C3-33) [NBP2-80143] - ICC/IF analysis of rat brain hippocampus tissue shows that Ryanodine Receptor 2 Antibody (C3-33) crossreacts with Ryanodine Receptor 2.

Applications for Ryanodine Receptor 2 Antibody (C3-33) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:000

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1 ug/ml

Immunohistochemistry-Frozen

1 ug/ml

Immunohistochemistry-Paraffin

1 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

1 ug/ml
Application Notes
WB: Detects an approx. 565 kDa protein representing the ryanodine receptor. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor.

IHC: Staining of RyR in rat cardiac tissue results in intense staining of the myofiber, which is consistent with sarcoplasmic reticulum localization.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Background: Ryanodine Receptor 2

The Ryanodine Receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR 1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR 2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).

Long Name

Ryanodine receptor 2

Alternate Names

hRYR-2, RYR-2, RyR2

Gene Symbol

RYR2

Additional Ryanodine Receptor 2 Products

Product Documents for Ryanodine Receptor 2 Antibody (C3-33) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ryanodine Receptor 2 Antibody (C3-33) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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