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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI10D5

Concentration

1 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human S100A9 (NP_002956) produced in SF9 cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

13.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for S100A9 Antibody (OTI10D5)

Western Blot: S100A9 Antibody (OTI10D5) [NBP2-45662]

Western Blot: S100A9 Antibody (OTI10D5) [NBP2-45662]

Western Blot: S100A9 Antibody (10D5) [NBP2-45662] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100A9 .
Immunohistochemistry: S100A9 Antibody (OTI10D5) [NBP2-45662]

Immunohistochemistry: S100A9 Antibody (OTI10D5) [NBP2-45662]

Immunohistochemistry: S100A9 Antibody (10D5) [NBP2-45662] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: S100A9 Antibody (OTI10D5) [NBP2-45662]

Immunohistochemistry: S100A9 Antibody (OTI10D5) [NBP2-45662]

Immunohistochemistry: S100A9 Antibody (10D5) [NBP2-45662] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for S100A9 Antibody (OTI10D5)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: S100A9

S100A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A9 may function in the inhibition of casein kinase, and altered expression is associated with the disease cystic fibrosis. Overexpression of S100A9 has also recently been linked to a poor prognosis with invasive ductal carcinoma of the breast.

Long Name

S100 Calcium Binding Protein A9

Alternate Names

Calgranulin B, MRP-14

Gene Symbol

S100A9

Additional S100A9 Products

Product Documents for S100A9 Antibody (OTI10D5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for S100A9 Antibody (OTI10D5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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