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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI10D5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human S100A9 (NP_002956) produced in SF9 cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

13.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for S100A9 Antibody (OTI10D5) - Azide and BSA Free

Western Blot: S100A9 Antibody (OTI10D5)Azide and BSA Free [NBP2-73970]

Western Blot: S100A9 Antibody (OTI10D5)Azide and BSA Free [NBP2-73970]

Western Blot: S100A9 Antibody (OTI10D5) - Azide and BSA Free [NBP2-73970] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100A9 .
Immunohistochemistry: S100A9 Antibody (OTI10D5) - Azide and BSA Free [NBP2-73970]

Immunohistochemistry: S100A9 Antibody (OTI10D5) - Azide and BSA Free [NBP2-73970]

Immunohistochemistry: S100A9 Antibody (OTI10D5) - Azide and BSA Free [NBP2-73970] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: S100A9 Antibody (OTI10D5) - Azide and BSA Free [NBP2-73970]

Immunohistochemistry: S100A9 Antibody (OTI10D5) - Azide and BSA Free [NBP2-73970]

Immunohistochemistry: S100A9 Antibody (OTI10D5) - Azide and BSA Free [NBP2-73970] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for S100A9 Antibody (OTI10D5) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: S100A9

S100A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A9 may function in the inhibition of casein kinase, and altered expression is associated with the disease cystic fibrosis. Overexpression of S100A9 has also recently been linked to a poor prognosis with invasive ductal carcinoma of the breast.

Long Name

S100 Calcium Binding Protein A9

Alternate Names

Calgranulin B, MRP-14

Gene Symbol

S100A9

Additional S100A9 Products

Product Documents for S100A9 Antibody (OTI10D5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for S100A9 Antibody (OTI10D5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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