SCP1 Antibody - BSA Free

Immunohistochemistry-Paraffin: SCP1 Antibody - BSA Free [NB300-229]

Immunohistochemistry-Paraffin: SCP1 Antibody [NB300-229] - Punctate staining of murine SCP1 in mouse ovary using NB300-229.

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229]

Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - SCP1 labeled in mouse pachytene preparation (red), using NB300-229. CDK2 staining, near teleomeres, is also present (green).

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229]

Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - Spermatocytes cells fixed in PFA. Detected with anti-mouse 594. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229]

Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - Mouse spermatozoa. Green: SCP1 staining. ICC/IF image submitted by a verified customer review.

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229]

Immunocytochemistry/Immunofluorescence: SCP1 Antibody [NB300-229] - Mouse spermatocyte. Red: scp1 colocalized with scp3 (green). ICC/IF image submitted by a verified customer review.

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229]

Immunocytochemistry/Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - Immunostaining of SCP3/SYCP3 (NB300-231) and SCP1 on adult Cxxc1 het and CKO chromosome spreads. Green, SCP3/SYCP3; orange, SCP1. Scale bar, 10 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30365547/) licensed under a CC-BY license.

Chromatin Immunoprecipitation: SCP1 Antibody - BSA Free [NB300-229] -

Chromatin Immunoprecipitation: SCP1 Antibody - BSA Free [NB300-229] - Meiotic spreads.Meiotic chromosome spreads from wild type & Fance mutant (MT) primary spermatocytes isolated from the testes at 20 dpp. Each chromatin spread was staged by analyzing SYCP3 (red) & SYCP1 (green), which are axial element & central region components of the synaptonemal complex respectively. Most of the Fance mutant spermatocytes displayed normal synapsis (middle panels); however a sub-set displayed synapsis abnormalities including an association between non-homologous chromosome ends (arrow head) & abnormal SYCP3 structures (arrows). Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0159800), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] -

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - Meiotic progression & chromosome paring in XX, XO & XY oocytes.(A) Meiotic progression in XX, XO & XY oocytes. The graphs show the percentages of the meiotic stage at the day of culture indicated. L, leptotene; Z, zygotene; P, pachytene; D, diplotene. (B) Pairing of homologous chromosomes in XX, XO & XY oocytes. Images show the immunofluorescence analysis of SYCP3 (white) & SYCP1 (red), & FISH analysis of the X chromosome (green) & Y chromosome (purple). The dashed squares in the merged images are shown at high magnification (right). The numbers of samples showing the phenotype are shown with the total number tested (left). Arrowheads indicate asynapsed bivalents at the end of the chromosomes. Scale bars, 10 μm. (C) Pairing rates of autosomes & sex chromosomes. Each value was calculated from three independent experiments (see also Materials & Methods). P values were calculated by Tukey’s HSD test. ***P<0.001, **P<0.01, *P<0.05; NS, not significant. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32214314), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] -

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - Meiotic prophase I progression & apoptosis in spermatocytes in ex vivo culture at different temperatures.a Representative images of chromosomal spreads at different stages of meiotic prophase I, prepared from in vivo-developed testes of 5-week-old Acr-GFP mice & from testis explants cultured at 34, 37, or 38 °C for 5 weeks as indicated. Samples were stained for SCP3, SCP1, & DNA (Hoechst 33342). Scale bars, 10 μm. b Proportions of spermatocytes in the leptotene (lep), zygotene (zygo), pachytene (pachy), & diplotene (diplo) stages, found in explants cultured for 5 weeks at the indicated temperatures classified visually on the chromosome spreads after immunofluorescence-staining for SCP1 & SCP3 as (a), according to the criteria described in the text. Values obtained from chromosome spreads prepared from pooled testicular cells of two in vivo-developed testes from different individuals & those from 6 to 7 ex vivo-grown explants are summarized. The total number of spermatocyte nuclei counted is indicated at the upper right of each panel. Percentages of spermatocytes nuclei in which all the autosomes have completed synapsis (i.e., the sum of pachytene & diplotene spermatocytes) were 63, 52, 16, & 0.5% for in vivo & ex vivo samples at 34, 37, & 38 °C, respectively. c Detection of cleaved Caspase-3 (red) & SCP3 (green) in testis explants following the temperature shift from 34 to 38 °C. Double-stained images overlaid with DNA staining (gray) & the signals for cleaved Caspase-3 alone are shown in the upper & lower panels, respectively. Enlarged images at positions indicated by rectangles are also shown below. Yellow arrowheads, Caspase-3+/SCP3+ double-positive cells (the dying spermatocytes). Scale bars, 40 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35618762), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] -

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - No major meiotic DSB repair or chromosome synapsis defects are observed in Cxxc1 CKO testis.(A) Immunostaining of SYCP3 & gammaH2AX on adult Cxxc1 het & CKO chromosome spreads. Green, SYCP3; magenta, gammaH2AX. Scale bar, 10 μm. (B) Spermatocyte stage proportion in adult Cxxc1 het (n = 1,062 from two individuals) & CKO (n = 1,105 from two individuals) spermatocytes based on SYCP3/SYCP1/ gammaH2AX staining. p = 0.7 by Chi-square test. (C) Immunostaining of SYCP3 & SYCP1 on adult Cxxc1 het & CKO chromosome spreads. Green, SYCP3; orange, SYCP1. Scale bar, 10 μm. (D) Crossover number measured by MLH1 staining on chromosome spreads of adult Cxxc1 het & CKO spermatocytes. Left, magenta, SYCP3; green, MLH1. Scale bar, 10 μm. Right, number of MLH1 foci per late pachynema in Cxxc1 het (n = 32 from two individuals) & CKO (n = 33 from two individuals). Bars represent mean ± SD. p = 0.4 by Student’s t-test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30365547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] -

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody - BSA Free [NB300-229] - UHRF1 deletion disrupted the meiotic progression & synaptonemal complex assembly.a Relative amounts of four spermatocyte populations (leptotene stage, zygotene stage, pachytene stage, & diplotene stage) during the prophase I in testes based on analyzing >600 spermatocytes in each stage. b, c The immunostaining of SYCP3 in the testicular sections (b) & surface-spread chromatin preparations of Uhrf1 deletion & control mice (c); d the percentage of spermatocytes with abnormal SYCP3 location. e Double immunofluorescence of testicular spread preparations of the adult mice, SYCP3 (green) & SYCP1 (red). f The percentage of spermatocytes with abnormal SYCP1 location. Lep leptotene, Zyg zygotene, Pac pachytene, Dip diplotene. Data are presented as mean ± SEM of three mice. ***p ≤ 0.001. Scale bar, 25 μm in b, 5 μm in c, e. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32081844), licensed under a CC-BY license. Not internally tested by Novus Biologicals.