SCP1 Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NB300-229B
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse, Rat, Chicken, Human (Negative), Mammal, Monkey, Parasite
Cited:
Mouse
Applications
Validated:
Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Biotin
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide made to the C-terminus of the mouse SCP1 protein sequence. [UniProt# Q62209]
Reactivity Notes
Mammal reactivity reported in scientific literature (PMID: 25981592). Parasite reactivity reported in scientific literature (PMID: 27084479). Chicken reactivity reported in scientific literature (PMID: 28174243). Use in Monkey reported in scientific literature (PMID: 31907447). This antibody has not been shown to have human reactivity.
Localization
Nuclear
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SCP1 Antibody [Biotin]
Immunocytochemistry/Immunofluorescence: SCP1 Antibody [Biotin] [NB300-229B] - Immunofluorescence staining of spermatocyte nuclei. Immunofluorescence images and signal quantification of stage-specific spermatocyte nuclei through meiosis prophase I. Details for signal quantification are described in Methods. Microscopic images are selected from two independent experiments in which two different combinations of primary antibodies are used; one using SCP3, H1t and SCP1, another one using SCP3 and STRA8. *Early and late pachytene nuclei cannot be unambiguously differentiated in the absence of H1t staining, and are therefore merged for counting and signal quantification. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-11820-7), licensed under a CC-BY license.
Immunocytochemistry/Immunofluorescence: SCP1 Antibody [Biotin] [NB300-229B] - Immunofluorescence staining of spermatocyte nuclei. Immunofluorescence images and signal quantification of stage-specific spermatocyte nuclei through meiosis prophase I. Details for signal quantification are described in Methods. Microscopic images are selected from two independent experiments in which two different combinations of primary antibodies are used; one using SCP3, H1t and SCP1, another one using SCP3 and STRA8. *Early and late pachytene nuclei cannot be unambiguously differentiated in the absence of H1t staining, and are therefore merged for counting and signal quantification. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-11820-7), licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [Biotin] [NB300-229B] -
Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [Biotin] [NB300-229B] - Immunofluorescence staining of spermatocyte nuclei. Immunofluorescence images & signal quantification of stage-specific spermatocyte nuclei through meiosis prophase I. Details for signal quantification are described in Methods. Microscopic images are selected from two independent experiments in which two different combinations of primary antibodies are used; one using SCP3, H1t & SCP1, another one using SCP3 & STRA8. *Early & late pachytene nuclei cannot be unambiguously differentiated in the absence of H1t staining, & are therefore merged for counting & signal quantification. Source data are provided as a Source Data file Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31444359), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for SCP1 Antibody [Biotin]
Application
Recommended Usage
Chromatin Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Chromatin Immunoprecipitation (ChIP)
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Simple Western
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: SCP1
Alternate Names
Cancer/testis antigen 8, CT8MGC104417, HOM-TES-14, SCP1SCP-1, synaptonemal complex protein 1
Gene Symbol
SYCP1
Additional SCP1 Products
Product Documents for SCP1 Antibody [Biotin]
Product Specific Notices for SCP1 Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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