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SCP1 Antibody [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NB300-229B

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse, Rat, Chicken, Human (Negative), Mammal, Monkey, Parasite

Cited:

Mouse

Applications

Validated:

Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide made to the C-terminus of the mouse SCP1 protein sequence. [UniProt# Q62209]

Reactivity Notes

Mammal reactivity reported in scientific literature (PMID: 25981592). Parasite reactivity reported in scientific literature (PMID: 27084479). Chicken reactivity reported in scientific literature (PMID: 28174243). Use in Monkey reported in scientific literature (PMID: 31907447). This antibody has not been shown to have human reactivity.

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SCP1 Antibody [Biotin]

Immunocytochemistry/Immunofluorescence: SCP1 Antibody [Biotin] [NB300-229B] - Immunofluorescence staining of spermatocyte nuclei. Immunofluorescence images and signal quantification of stage-specific spermatocyte nuclei through meiosis prophase I. Details for signal quantification are described in Methods. Microscopic images are selected from two independent experiments in which two different combinations of primary antibodies are used; one using SCP3, H1t and SCP1, another one using SCP3 and STRA8. *Early and late pachytene nuclei cannot be unambiguously differentiated in the absence of H1t staining, and are therefore merged for counting and signal quantification. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-11820-7), licensed under a CC-BY license.
Immunocytochemistry/Immunofluorescence: SCP1 Antibody [Biotin] [NB300-229B] - Immunofluorescence staining of spermatocyte nuclei. Immunofluorescence images and signal quantification of stage-specific spermatocyte nuclei through meiosis prophase I. Details for signal quantification are described in Methods. Microscopic images are selected from two independent experiments in which two different combinations of primary antibodies are used; one using SCP3, H1t and SCP1, another one using SCP3 and STRA8. *Early and late pachytene nuclei cannot be unambiguously differentiated in the absence of H1t staining, and are therefore merged for counting and signal quantification. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-11820-7), licensed under a CC-BY license.
SCP1 Antibody [Biotin]

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [Biotin] [NB300-229B] -

Immunocytochemistry/ Immunofluorescence: SCP1 Antibody [Biotin] [NB300-229B] - Immunofluorescence staining of spermatocyte nuclei. Immunofluorescence images & signal quantification of stage-specific spermatocyte nuclei through meiosis prophase I. Details for signal quantification are described in Methods. Microscopic images are selected from two independent experiments in which two different combinations of primary antibodies are used; one using SCP3, H1t & SCP1, another one using SCP3 & STRA8. *Early & late pachytene nuclei cannot be unambiguously differentiated in the absence of H1t staining, & are therefore merged for counting & signal quantification. Source data are provided as a Source Data file Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31444359), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for SCP1 Antibody [Biotin]

Application
Recommended Usage

Chromatin Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: SCP1

The synaptonemal complex is a proteinaceous complex that apparently mediates synapses during the zygotene stage and then disintegrates. It is a complex structure that unites homologous chromosomes during the prophase stage of meiosis. It is the result of chromosome pairing interactions and not the cause of them.

Alternate Names

Cancer/testis antigen 8, CT8MGC104417, HOM-TES-14, SCP1SCP-1, synaptonemal complex protein 1

Gene Symbol

SYCP1

Additional SCP1 Products

Product Documents for SCP1 Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SCP1 Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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