SDHB Antibody (CL0346)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30677

Novus Biologicals, part of Bio-Techne
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NBP2-30677
NBP2-30677-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL0346

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SDHB Antibodies »

Product Summary for SDHB Antibody (CL0346)

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: EGKQQYLQSIEEREKLDGLYECILCACCSTSCPSYWWNGDKYLGPAVLMQAYRWMIDSRDDFTEERLAKLQDPFSLYRCHTIMNCTRTCPKGLNPGKAIAEIKKMMATY

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Marker

Mitochondria Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for SDHB Antibody (CL0346)

Western Blot: SDHB Antibody (CL0346) [NBP2-30677]

Western Blot: SDHB Antibody (CL0346) [NBP2-30677]

Western Blot: SDHB Antibody (CL0346) [NBP2-30677] - Lane 1: Marker [kDa]. Lane 2: Human cell line RT-4
Immunohistochemistry: SDHB Antibody (CL0346) [NBP2-30677]

Immunohistochemistry: SDHB Antibody (CL0346) [NBP2-30677]

Immunohistochemistry: SDHB Antibody (CL0346) [NBP2-30677] - Staining of human testis shows moderate cytoplasmic immunoreactivity in the seminiferous tubules.
Immunohistochemistry: SDHB Antibody (CL0346) [NBP2-30677]

Immunohistochemistry: SDHB Antibody (CL0346) [NBP2-30677]

Immunohistochemistry: SDHB Antibody (CL0346) [NBP2-30677] - Staining of human liver shows moderate cytoplasmic positivity in the hepatocytes.
View 2 more images

Applications for SDHB Antibody (CL0346)

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SDHB

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq]

Alternate Names

EC 1.3.5.1, FLJ92337, Ip, Iron-sulfur subunit of complex II, PGL4, SDH, SDH1, SDH2, SDHIP, succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, succinate dehydrogenase complex, subunit B, iron sulfur (Ip)

Gene Symbol

SDHB

UniProt

P21912 (Human)

Additional SDHB Products

Product Documents for SDHB Antibody (CL0346)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SDHB Antibody (CL0346)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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