SDHB Antibody (CL0346) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43913

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL0346

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SDHB Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P21912, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for SDHB Antibody (CL0346) - Azide and BSA Free

SDHB Antibody (CL0346) - Azide and BSA Free Western Blot: SDHB Antibody [NBP3-43913]

Western Blot: SDHB Antibody [NBP3-43913]

Lane 1: Marker [kDa]
Lane 2: Human cell line RT-4
SDHB Antibody (CL0346) - Azide and BSA Free Immunohistochemistry-Paraffin: SDHB Antibody [NBP3-43913]

Immunohistochemistry-Paraffin: SDHB Antibody [NBP3-43913]

Staining of human heart shows strong cytoplasmic positivity in cardiomyocytes.
SDHB Antibody (CL0346) - Azide and BSA Free Immunohistochemistry-Paraffin: SDHB Antibody [NBP3-43913]

Immunohistochemistry-Paraffin: SDHB Antibody [NBP3-43913]

Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
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Applications for SDHB Antibody (CL0346) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SDHB

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq]

Alternate Names

EC 1.3.5.1, FLJ92337, Ip, Iron-sulfur subunit of complex II, PGL4, SDH, SDH1, SDH2, SDHIP, succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, succinate dehydrogenase complex, subunit B, iron sulfur (Ip)

Gene Symbol

SDHB

Additional SDHB Products

Product Documents for SDHB Antibody (CL0346) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SDHB Antibody (CL0346) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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