Serpin E1/PAI-1 Antibody (10C3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26327

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10C3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Serpin E1/PAI-1 Antibodies »

Product Summary for Serpin E1/PAI-1 Antibody (10C3)

Immunogen

A synthesized peptide derived from Human Serpin E1/PAI-1 [UniProt P05121]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Serpin E1/PAI-1 Antibody (10C3)

Serpin E1/PAI-1 Antibody (10C3)

Immunohistochemistry: Serpin E1/PAI-1 Antibody (10C3) [NBP3-26327] -

Immunohistochemistry: Serpin E1/PAI-1 Antibody (10C3) [NBP3-26327] - Image of Serpin E1/PAI-1 Antibody (10C3) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Serpin E1/PAI-1 Antibody (10C3)

Western Blot: Serpin E1/PAI-1 Antibody (10C3) [NBP3-26327] -

Western Blot: Serpin E1/PAI-1 Antibody (10C3) [NBP3-26327] - Positive Western Blot detected in: U-251 whole cell lysate, U-87 whole cell lysate.
All lanes: PAI1 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 46, 44 kDa
Observed band size: 45 kDa

Applications for Serpin E1/PAI-1 Antibody (10C3)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Serpin E1/PAI-1

PAI-1 and PAI-2 (for plasminogen activator inhibitor-1 and -2) are members of the serpin serine proteinase inhibitor family. PAI-1 and PAI-2 have been shown to regulate uPA (urokinase-type plasminogen activator) and tPA (tissue plasminogen activator), resulting in the inhibition of proteolytic activity. Members of the serpin family generally complex with their target proteinases, then disassociate slowly into cleaved species that fold into stable inactive forms. PAI-1 can fold into the inactive state without cleavage, resulting in the latent form of PAI-1. Activity can be restored to the latent form of PAI-1 through denaturation and renaturation. PAI-2 occurs in secreted and cytosolic forms through facultative polypeptide translocation. uPA is a serine proteinase that is a member of the trypsin family. It is responsible for the cleavage of plasminogen at the Arg-Val bond to produce plasmin. uPA consists of two chains designated A and B. The A chain can be cleaved, resulting in low and high molecular mass forms of uPA.

Long Name

Plasminogen Activator Inhibitor

Alternate Names

Nexin, PAI-1, PAI1, PLANH1

Gene Symbol

SERPINE1

Additional Serpin E1/PAI-1 Products

Product Documents for Serpin E1/PAI-1 Antibody (10C3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Serpin E1/PAI-1 Antibody (10C3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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