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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

ELISA, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human PAI1/Serpine 1 protein (between residues 300-400) [UniProt P05121]

Reactivity Notes

Use in Rat reported in scientific literature (PMID:35386330).

Localization

Secreted.

Specificity

PAI-1 (N315) pAb detects endogenous levels of PAI-1 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Serpin E1/PAI-1 Antibody - BSA Free

Western Blot: Serpin E1/PAI-1 AntibodyBSA Free [NBP1-19773]

Western Blot: Serpin E1/PAI-1 AntibodyBSA Free [NBP1-19773]

Western Blot: Serpin E1/PAI-1 Antibody [NBP1-19773] - Extracts from Jurkat cells.
Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody - BSA Free [NBP1-19773]

Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody - BSA Free [NBP1-19773]

Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody [NBP1-19773] - Staining of PAI1/Serpine 1 in mouse pancreas.
Western Blot: Serpin E1/PAI-1 AntibodyBSA Free [NBP1-19773]

Western Blot: Serpin E1/PAI-1 AntibodyBSA Free [NBP1-19773]

Western Blot: Serpin E1/PAI-1 Antibody - BSA Free [NBP1-19773] - Serpin E1/PAI-1 Antibody [NBP1-19773] - Western Blot: Serpin E1/PAI-1 Antibody [NBP1-19773] - Immunoblots showing protein expression of ALK5, pSMAD2/3, PAI-1, pVHL, andI2-actin in A498 cells after treatment with TGF-i2 at given point of time. Image collected and cropped by Citeab from the following publication (VHL status regulates transforming growth factor B signaling pathways in renal cell carcinoma. Oncotarget (2018)) licensed under a CC-BY license.

Applications for Serpin E1/PAI-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 - 10 ug/ml

Immunohistochemistry

1:50 - 1:100

Immunohistochemistry-Frozen

1:50 - 1:100

Immunohistochemistry-Paraffin

1:50 - 1:100

Western Blot

1.0 ug/ml

Reviewed Applications

Read 3 reviews rated 4.3 using NBP1-19773 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Serpin E1/PAI-1

PAI-1 and PAI-2 (for plasminogen activator inhibitor-1 and -2) are members of the serpin serine proteinase inhibitor family. PAI-1 and PAI-2 have been shown to regulate uPA (urokinase-type plasminogen activator) and tPA (tissue plasminogen activator), resulting in the inhibition of proteolytic activity. Members of the serpin family generally complex with their target proteinases, then disassociate slowly into cleaved species that fold into stable inactive forms. PAI-1 can fold into the inactive state without cleavage, resulting in the latent form of PAI-1. Activity can be restored to the latent form of PAI-1 through denaturation and renaturation. PAI-2 occurs in secreted and cytosolic forms through facultative polypeptide translocation. uPA is a serine proteinase that is a member of the trypsin family. It is responsible for the cleavage of plasminogen at the Arg-Val bond to produce plasmin. uPA consists of two chains designated A and B. The A chain can be cleaved, resulting in low and high molecular mass forms of uPA.

Long Name

Plasminogen Activator Inhibitor

Alternate Names

Nexin, PAI-1, PAI1, PLANH1

Entrez Gene IDs

5054 (Human); 18787 (Mouse); 24617 (Rat)

Gene Symbol

SERPINE1

UniProt

Additional Serpin E1/PAI-1 Products

Product Documents for Serpin E1/PAI-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Serpin E1/PAI-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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