Serpin E1/PAI-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13298

Novus Biologicals, part of Bio-Techne
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NBP2-13298
NBP2-13298-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Serpin E1/PAI-1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: RLFHKSDGSTVSVPMMAQTNKFNYTEFTTPDGHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPKFSLETEVDLRKPLENLGMTDMFRQFQADF

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (84%). Human reactivity reported in scientific literature (PMID: 25387487).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Serpin E1/PAI-1 Antibody

Immunocytochemistry/ Immunofluorescence: Serpin E1/PAI-1 Antibody [NBP2-13298]

Immunocytochemistry/ Immunofluorescence: Serpin E1/PAI-1 Antibody [NBP2-13298]

Immunocytochemistry/Immunofluorescence: Serpin E1/PAI-1 Antibody [NBP2-13298] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody [NBP2-13298]

Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody [NBP2-13298]

Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody [NBP2-13298] - Staining of human urinary bladder shows moderate cytoplasmic positivity in a subset of urothelial cells.
Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody [NBP2-13298]

Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody [NBP2-13298]

Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody [NBP2-13298] - Staining of human colon shows strong cytoplasmic positivity in endothelial cells.
View 2 more images

Applications for Serpin E1/PAI-1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Serpin E1/PAI-1

PAI-1 and PAI-2 (for plasminogen activator inhibitor-1 and -2) are members of the serpin serine proteinase inhibitor family. PAI-1 and PAI-2 have been shown to regulate uPA (urokinase-type plasminogen activator) and tPA (tissue plasminogen activator), resulting in the inhibition of proteolytic activity. Members of the serpin family generally complex with their target proteinases, then disassociate slowly into cleaved species that fold into stable inactive forms. PAI-1 can fold into the inactive state without cleavage, resulting in the latent form of PAI-1. Activity can be restored to the latent form of PAI-1 through denaturation and renaturation. PAI-2 occurs in secreted and cytosolic forms through facultative polypeptide translocation. uPA is a serine proteinase that is a member of the trypsin family. It is responsible for the cleavage of plasminogen at the Arg-Val bond to produce plasmin. uPA consists of two chains designated A and B. The A chain can be cleaved, resulting in low and high molecular mass forms of uPA.

Long Name

Plasminogen Activator Inhibitor

Alternate Names

Nexin, PAI-1, PAI1, PLANH1

Gene Symbol

SERPINE1

Additional Serpin E1/PAI-1 Products

Product Documents for Serpin E1/PAI-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Serpin E1/PAI-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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