SHP-2/PTPN11 Antibody (OTI3F2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03063

Novus Biologicals, part of Bio-Techne
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NBP2-03063
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3F2

Concentration

0.99 mg/ml

View all SHP-2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PTPN11(NP_002825) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

67.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SHP-2/PTPN11 Antibody (OTI3F2)

Western Blot: PTPN11/SHP2 Antibody (3F2) [NBP2-03063] Analysis of extracts (35ug) from 9 different cell lines by using anti-PTPN11/SHP2 monoclonal antibody.
Flow Cytometry: SHP-2/PTPN11 Antibody (OTI3F2) [NBP2-03063]

Flow Cytometry: SHP-2/PTPN11 Antibody (OTI3F2) [NBP2-03063]

Flow Cytometry: PTPN11/SHP2 Antibody (3F2) [NBP2-03063] - Analysis of Jurkat cells, using anti-PTPN11/SHP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: SHP-2/PTPN11 Antibody (OTI3F2) [NBP2-03063]

Western Blot: SHP-2/PTPN11 Antibody (OTI3F2) [NBP2-03063]

Western Blot: PTPN11/SHP2 Antibody (3F2) [NBP2-03063] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN11/SHP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN11/SHP2.
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Applications for SHP-2/PTPN11 Antibody (OTI3F2)

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.99 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SHP-2

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq].

Long Name

Src Homology Region 2 Domain Phosphatase 2

Alternate Names

PTP1D, PTP2C, PTPN11, SH-PTP2, SHP2

Entrez Gene IDs

5781 (Human)

Gene Symbol

PTPN11

Additional SHP-2 Products

Product Documents for SHP-2/PTPN11 Antibody (OTI3F2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SHP-2/PTPN11 Antibody (OTI3F2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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