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Siglec-8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06078

Novus Biologicals, part of Bio-Techne
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NBP1-06078

Key Product Details

Species Reactivity

Human

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-SYKSQLNYKTKQ corresponding to internal region according to NP_055257.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Siglec-8 Antibody

Western Blot: Siglec-8 Antibody [NBP1-06078]

Western Blot: Siglec-8 Antibody [NBP1-06078]

Western Blot: Siglec-8 Antibody [NBP1-06078] - (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Siglec-8 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 45 kDa band observed in MOLT4 lysates is reported in scientific literature (PMID: 12609831).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Siglec-8

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface

Long Name

Sialic Acid Binding Ig-like Lectin 8

Alternate Names

SAF2, Siglec8, SIGLEC8L

Entrez Gene IDs

27181 (Human)

Gene Symbol

SIGLEC8

UniProt

Additional Siglec-8 Products

Product Documents for Siglec-8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Siglec-8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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