Siglec-8 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31141
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Siglec-8 Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Siglec-8. The exact sequence is proprietary.
Localization
Membrane, Single-pass type I membrane protein
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Siglec-8 Antibody
![Western Blot: Siglec-8 Antibody [NBP1-31141]](https://resources.bio-techne.com/images/products/Siglec-8-Antibody-Western-Blot-NBP1-31141-img0006.jpg "Western Blot: Siglec-8 Antibody [NBP1-31141]")
Western Blot: Siglec-8 Antibody [NBP1-31141]
Western Blot: Siglec-8 Antibody [NBP1-31141] - A: A431 7.5% SDS PAGE SIGLEC8 antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: Siglec-8 Antibody [NBP1-31141]](https://resources.bio-techne.com/images/products/Siglec-8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31141-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Siglec-8 Antibody [NBP1-31141]")
Immunocytochemistry/ Immunofluorescence: Siglec-8 Antibody [NBP1-31141]
Immunocytochemistry/Immunofluorescence: Siglec-8 Antibody [NBP1-31141] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.![Immunohistochemistry-Paraffin: Siglec-8 Antibody [NBP1-31141]](https://resources.bio-techne.com/images/products/Siglec-8-Antibody-Immunohistochemistry-Paraffin-NBP1-31141-img0005.jpg "Immunohistochemistry-Paraffin: Siglec-8 Antibody [NBP1-31141]")
Immunohistochemistry-Paraffin: Siglec-8 Antibody [NBP1-31141]
Immunohistochemistry-Paraffin: Siglec-8 Antibody [NBP1-31141] - Human breast cancer, using SIGLEC8 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15minApplications for Siglec-8 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Siglec-8
Long Name
Sialic Acid Binding Ig-like Lectin 8
Alternate Names
SAF2, Siglec8, SIGLEC8L
Gene Symbol
SIGLEC8
Additional Siglec-8 Products
Product Documents for Siglec-8 Antibody
Product Specific Notices for Siglec-8 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.