SIRP alpha/CD172a Antibody (4C7)

Novus Biologicals, part of Bio-Techne | Catalog # H00140885-M10

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4C7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SIRP alpha/CD172a Antibodies »

Product Specifications

Immunogen

SIRPA (AAH26692, 28 a.a. ~ 507 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VAGEEELQVIQPDKSVSVAAGESAILHCTVTSLIPVGPIQWFRGAGPARELIYNQKEGHFPRVTTVSESTKRENMDFSISISNITPADAGTYYCVKFRKGSPDTEFKSGAGTELSVRAKPSAPVVSGPAARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNERNIYIVVGVVCTLLVALLMAALYLVRIRQKKAQGSTSSTRLHEPEKNAREITQVQSLDTNDITYADLNLPKGKKPAPQAAEPNNHTEYASIQTSPQPASEDTLTYADLDMVHLNRTPKQPAPKPEPSFSEYASVQVPRK

Specificity

Protein tyrosine phosphatase, non-receptor type substrate 1. This antibody was made to an older known protein sequence of human SIRPA. This sequence shares 96% homology to the currently accepted sequence for human SIRPA (accession number P78324) found on Uniprot.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SIRP alpha/CD172a Antibody (4C7)

Western Blot: SIRP alpha/CD172a Antibody (4C7) [H00140885-M10]

Western Blot: SIRP alpha/CD172a Antibody (4C7) [H00140885-M10]

Western Blot: SIRP alpha/CD172a Antibody (4C7) [H00140885-M10] - Analysis of SIRPA expression in Raw 264.7 (Cat # L024V1).
Immunocytochemistry/ Immunofluorescence: SIRP alpha/CD172a Antibody (4C7) [H00140885-M10]

Immunocytochemistry/ Immunofluorescence: SIRP alpha/CD172a Antibody (4C7) [H00140885-M10]

Immunocytochemistry/Immunofluorescence: SIRP alpha/CD172a Antibody (4C7) [H00140885-M10] - Analysis of monoclonal antibody to SIRPA on NIH/3T3 cell. Antibody concentration 10 ug/ml
Western Blot: SIRP alpha/CD172a Antibody (4C7) [H00140885-M10]

Western Blot: SIRP alpha/CD172a Antibody (4C7) [H00140885-M10]

Western Blot: SIRP alpha/CD172a Antibody (4C7) [H00140885-M10] - Analysis of SIRPA expression in NIH/3T3 (Cat # L018V1).
View 1 more images

Applications for SIRP alpha/CD172a Antibody (4C7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SIRP alpha/CD172a

The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene.

Long Name

Signal-regulatory Protein alpha

Alternate Names

BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA

Entrez Gene IDs

140885 (Human)

Gene Symbol

SIRPA

OMIM

602461 (Human)

UniProt

AAH26692 (Human)

Additional SIRP alpha/CD172a Products

Product Documents for SIRP alpha/CD172a Antibody (4C7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SIRP alpha/CD172a Antibody (4C7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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