SIRP alpha/CD172a Antibody (JE36-20)

Western Blot: SIRP alpha/CD172a Antibody (JE36-20) [NBP3-32950]

Western Blot: SIRP alpha/CD172a Antibody (JE36-20) [NBP3-32950] - Western blot analysis of SIRP alpha/CD172a on different lysates with Rabbit anti-SIRP alpha/CD172a antibody (NBP3-32950) at 1/500 dilution.

Lane 1: THP-1 cell lysate (10 ug/Lane)
Lane 2: HL-60 cell lysate (10 ug/Lane)
Lane 3: Mouse brain tissue lysate (20 ug/Lane)

Predicted band size: 55 kDa
Observed band size: 75-100 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32950) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: SIRP alpha/CD172a Antibody (JE36-20) [NBP3-32950]

Immunohistochemistry: SIRP alpha/CD172a Antibody (JE36-20) [NBP3-32950] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-SIRP alpha/CD172a antibody (NBP3-32950) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32950) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: SIRP alpha/CD172a Antibody (JE36-20) [NBP3-32950]

Flow Cytometry: SIRP alpha/CD172a Antibody (JE36-20) [NBP3-32950] - Flow cytometric analysis of THP-1 cells labeling SIRP alpha/CD172a.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32950, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).