SIRP alpha/CD172a Antibody (OTI7B3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74197

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI7B3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SIRP alpha/CD172a Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SIRPA (NP_001035111) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SIRP alpha/CD172a Antibody (OTI7B3) - Azide and BSA Free

Western Blot: SIRP alpha/CD172a Antibody (OTI7B3)Azide and BSA Free [NBP2-74197]

Western Blot: SIRP alpha/CD172a Antibody (OTI7B3)Azide and BSA Free [NBP2-74197]

Western Blot: SIRP alpha/CD172a Antibody (OTI7B3) - Azide and BSA Free [NBP2-74197] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRP alpha.
Immunohistochemistry: SIRP alpha/CD172a Antibody (OTI7B3) - Azide and BSA Free [NBP2-74197]

Immunohistochemistry: SIRP alpha/CD172a Antibody (OTI7B3) - Azide and BSA Free [NBP2-74197]

Immunohistochemistry: SIRP alpha/CD172a Antibody (OTI7B3) - Azide and BSA Free [NBP2-74197] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)
Flow Cytometry: SIRP alpha/CD172a Antibody (OTI7B3) - Azide and BSA Free [NBP2-74197]

Flow Cytometry: SIRP alpha/CD172a Antibody (OTI7B3) - Azide and BSA Free [NBP2-74197]

Flow Cytometry: SIRP alpha/CD172a Antibody (OTI7B3) - Azide and BSA Free [NBP2-74197] - analysis of living A375 cells, using anti-SIRPA antibody , compared to an isotype control (green), and a PBS control (blue) (1:100).
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Applications for SIRP alpha/CD172a Antibody (OTI7B3) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SIRP alpha/CD172a

Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1), SHPS-1 (2,3), MyD-1 (4), BIT (5,6) and p84 (7). SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2 (1,2,5,8). SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes (1,2,5,8).

Long Name

Signal-regulatory Protein alpha

Alternate Names

BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA

Gene Symbol

SIRPA

Additional SIRP alpha/CD172a Products

Product Documents for SIRP alpha/CD172a Antibody (OTI7B3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SIRP alpha/CD172a Antibody (OTI7B3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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