Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # OX-41

Concentration

LYOPH mg/ml

View all SIRP alpha/CD172a Antibodies »

Product Specifications

Immunogen

Rat peritoneal macrophages

Reactivity Notes

Reacts with Rat. Use in Mouse reported in scientific literature (PMID:34413330)

Localization

Membrane; single pass type I membrane protein.

Specificity

NB100-65530 recognizes rat CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons. MRC OX-41 binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a - CD47.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for SIRP alpha/CD172a Antibody (OX-41) [FITC]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000
Application Notes
This product requires protein digestion pretreatment of paraffin sections e.g. trypsin or pronase.

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with 50 ul of distilled water to a final concentration is 1.0 mg/ml.

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SIRP alpha/CD172a

Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1), SHPS-1 (2,3), MyD-1 (4), BIT (5,6) and p84 (7). SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2 (1,2,5,8). SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes (1,2,5,8).

Long Name

Signal-regulatory Protein alpha

Alternate Names

BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA

Gene Symbol

SIRPA

UniProt

P97710

Additional SIRP alpha/CD172a Products

Product Documents for SIRP alpha/CD172a Antibody (OX-41) [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SIRP alpha/CD172a Antibody (OX-41) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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