SIRP alpha/CD172a Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20370

Novus Biologicals, part of Bio-Techne
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NBP2-20370
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SIRP alpha/CD172a Antibodies »

Product Summary for SIRP alpha/CD172a Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human SIRP alpha/CD172a. The exact sequence is proprietary.

Localization

Membrane; Single-pass type I membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SIRP alpha/CD172a Antibody

Western Blot: SIRP alpha/CD172a Antibody [NBP2-20370]

Western Blot: SIRP alpha/CD172a Antibody [NBP2-20370]

Western Blot: SIRP alpha/CD172a Antibody [NBP2-20370] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SIRP alpha antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: SIRP alpha/CD172a Antibody [NBP2-20370]

Immunocytochemistry/ Immunofluorescence: SIRP alpha/CD172a Antibody [NBP2-20370]

Immunocytochemistry/Immunofluorescence: SIRP alpha/CD172a Antibody [NBP2-20370] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SIRP alpha stained by SIRP alpha antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000.Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-20370]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-20370]

Immunohistochemistry-Paraffin: SIRP alpha/CD172a Antibody [NBP2-20370] - Human colon carcinoma, using SIRP alpha antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for SIRP alpha/CD172a Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay Dependent

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SIRP alpha/CD172a

The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq]

Long Name

Signal-regulatory Protein alpha

Alternate Names

BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA

Gene Symbol

SIRPA

UniProt

P78324

Additional SIRP alpha/CD172a Products

Product Documents for SIRP alpha/CD172a Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SIRP alpha/CD172a Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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