Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13878

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-SIRT3-1C10

Concentration

0.2 mg/ml

View all Sirtuin 3/SIRT3 Antibodies »

Product Summary for Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10)

Immunogen

Recombinant full-length human Sirtuin 3/SIRT3 protein (Uniprot: Q9NTG7)

Localization

Mitochondrion matrix

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

43.57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14289)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10)

Immunocytochemistry/ Immunofluorescence: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) [NBP3-13878]

Immunocytochemistry/ Immunofluorescence: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) [NBP3-13878]

Immunocytochemistry/Immunofluorescence: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) [NBP3-13878] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Flow Cytometry: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) [NBP3-13878]

Flow Cytometry: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) [NBP3-13878]

Flow Cytometry: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) [NBP3-13878] - Flow cytometric analysis of PFA-fixed HeLa cells. Sirtuin 3/SIRT3 antibody (PCRP-SIRT3-1C10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) [NBP3-13878]

Protein Array: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) [NBP3-13878]

Protein Array: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) [NBP3-13878] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10).

Applications for Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Sirtuin 3/SIRT3

SIRT3 is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).

Long Name

Silent Mating Type Information Regulation 3 Homolog

Alternate Names

SIR2-like protein 3, SIR2L3, SIRT3

Gene Symbol

SIRT3

UniProt

Q9NTG7 (Human)

Additional Sirtuin 3/SIRT3 Products

Product Documents for Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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