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Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14289

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-SIRT3-1C10

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant full-length human Sirtuin 3/SIRT3 protein (Uniprot: Q9NTG7)

Localization

Mitochondrion matrix

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

43.57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13878).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) - Azide and BSA Free

Protein Array: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) - Azide and BSA Free [NBP3-14289]

Protein Array: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) - Azide and BSA Free [NBP3-14289]

Protein Array: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) - Azide and BSA Free [NBP3-14289] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10).

Applications for Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug per 100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order this antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Sirtuin 3/SIRT3

SIRT3 is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).

Long Name

Silent Mating Type Information Regulation 3 Homolog

Alternate Names

SIR2-like protein 3, SIR2L3, SIRT3

Gene Symbol

SIRT3

Additional Sirtuin 3/SIRT3 Products

Product Documents for Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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