SLC6A3/DAT1 [p Thr53] Antibody

Validated:

Unconjugated

Polyclonal Rabbit IgG

Please see the vial label for concentration. If unlisted please contact technical services.

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 conjugated to KLH. Accession # P23977

p Thr53

Specific for endogenous levels of the ~55 kDa glycosylated form of the DAT protein phosphorylated at Thr53. Relative mobility may vary depending on the state of glycosylation of the DAT protein. The antibody works best in lysates that have not been boiled prior to being run on an SDS-PAGE gel. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Polyclonal

Rabbit

IgG

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Antigen Affinity-purified

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

No Preservative

Please see the vial label for concentration. If unlisted please contact technical services.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at -20C. Avoid freeze-thaw cycles.