SMAC/Diablo Antibody (006)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89251

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-89251-100ul
NBP2-89251-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 006

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SMAC/Diablo Antibodies »

Product Summary for SMAC/Diablo Antibody (006)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human SMAC/Diablo (Accession#: NP_063940.1; Ala 56-Asp 239).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for SMAC/Diablo Antibody (006)

Western Blot: SMAC/Diablo Antibody (006) [NBP2-89251]

Western Blot: SMAC/Diablo Antibody (006) [NBP2-89251]

Western Blot: SMAC/Diablo Antibody (006) [NBP2-89251] - Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell LysateLane C: A431 Whole Cell Lysate Lane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:20 kDa
Immunocytochemistry/ Immunofluorescence: SMAC/Diablo Antibody (006) [NBP2-89251]

Immunocytochemistry/ Immunofluorescence: SMAC/Diablo Antibody (006) [NBP2-89251]

Immunocytochemistry/Immunofluorescence: SMAC/Diablo Antibody (006) [NBP2-89251] - Staining of Human Diablo in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Diablo monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Flow Cytometry: SMAC/Diablo Antibody (006) [NBP2-89251]

Flow Cytometry: SMAC/Diablo Antibody (006) [NBP2-89251]

Flow Cytometry: SMAC/Diablo Antibody (006) [NBP2-89251] - Analysis of Human Diablo expression on HeLa cells. Stained with purified anti-Human Diablo (Filled hisgram), then a FITC-conjugated second step antibody. To demonstrate specificity of staining, the binding by was blocked by preincubation of the purified antibody with 20ug recombinant human Diablo for 1 hour (Black solid line hisgram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for SMAC/Diablo Antibody (006)

Application
Recommended Usage

ELISA

1:5000-1:10000

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Western Blot

1:10000-1:50000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMAC/Diablo

Smac/DIABLO is a mitochondrial protein which activates various forms of apoptosis. Specifically, Smac exits the mitochondria and enters the cytosol where it initiates apoptosis by neutralizing members of the IAP family of apoptosis inhibitory proteins. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms.

Long Name

Second Mitochondria-derived Activator of Caspase

Alternate Names

Diablo

Gene Symbol

DIABLO

Additional SMAC/Diablo Products

Product Documents for SMAC/Diablo Antibody (006)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SMAC/Diablo Antibody (006)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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