SMAC/Diablo Antibody (006)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89251
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 006
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human SMAC/Diablo (Accession#: NP_063940.1; Ala 56-Asp 239).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for SMAC/Diablo Antibody (006)
Western Blot: SMAC/Diablo Antibody (006) [NBP2-89251]
Western Blot: SMAC/Diablo Antibody (006) [NBP2-89251] - Lane A: Hela Whole Cell Lysate Lane B: Jurkat Whole Cell LysateLane C: A431 Whole Cell Lysate Lane D: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:22 kDaObserved band size:20 kDaImmunocytochemistry/ Immunofluorescence: SMAC/Diablo Antibody (006) [NBP2-89251]
Immunocytochemistry/Immunofluorescence: SMAC/Diablo Antibody (006) [NBP2-89251] - Staining of Human Diablo in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Diablo monoclonal antibody (1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.Flow Cytometry: SMAC/Diablo Antibody (006) [NBP2-89251]
Flow Cytometry: SMAC/Diablo Antibody (006) [NBP2-89251] - Analysis of Human Diablo expression on HeLa cells. Stained with purified anti-Human Diablo (Filled hisgram), then a FITC-conjugated second step antibody. To demonstrate specificity of staining, the binding by was blocked by preincubation of the purified antibody with 20ug recombinant human Diablo for 1 hour (Black solid line hisgram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.Applications for SMAC/Diablo Antibody (006)
Application
Recommended Usage
ELISA
1:5000-1:10000
Flow Cytometry
1:25-1:100
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Western Blot
1:10000-1:50000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SMAC/Diablo
Long Name
Second Mitochondria-derived Activator of Caspase
Alternate Names
Diablo
Gene Symbol
DIABLO
Additional SMAC/Diablo Products
Product Documents for SMAC/Diablo Antibody (006)
Product Specific Notices for SMAC/Diablo Antibody (006)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...