SMAC/Diablo Antibody - BSA Free

Western Blot: SMAC/Diablo AntibodyBSA Free [NBP1-76772]

Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Analysis of Smac in mouse heart tissue lysate in the absence (A) or presence (B) of blocking peptide and in rat heart tissue lysate with Smac antibody at 1 ug /ml.

Western Blot: SMAC/Diablo AntibodyBSA Free [NBP1-76772]

Western Blot: SMAC/Diablo Antibody [NBP1-76772] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: SMAC/Diablo NB100-55900 (1 ug/mL), SMAC/Diablo NBP1-76772 (1 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/ Immunofluorescence: SMAC/Diablo Antibody - BSA Free [NBP1-76772] -

Immunocytochemistry/ Immunofluorescence: SMAC/Diablo Antibody - BSA Free [NBP1-76772] - Figure 4 Immunofluorescence Validation of SMAC/Diablo in Mouse Spleen Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Spleen Cells labeling SMAC/Diablo with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Western Blot: SMAC/Diablo Antibody - BSA Free [NBP1-76772] -

Western Blot: SMAC/Diablo Antibody - BSA Free [NBP1-76772] - Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 u of lysates per lane.Antibodies: SMAC/Diablo 2409 (1 u/mL), SMAC/Diablo (1 u/mL), and beta-actin (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry: SMAC/Diablo Antibody - BSA Free [NBP1-76772] -

Immunohistochemistry: SMAC/Diablo Antibody - BSA Free [NBP1-76772] - Figure 5 Immunohistochemistry Validation of SMAC/Diablo in Mouse Spleen Tissue Immunohistochemical analysis of paraffin-embedded Mouse Spleen Tissue using anti-SMAC/Diablo antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry: SMAC/Diablo Antibody - BSA Free [NBP1-76772] -

Immunohistochemistry: SMAC/Diablo Antibody - BSA Free [NBP1-76772] - Figure 5 Immunohistochemistry Validation of SMAC/Diablo in Mouse Spleen Tissue
Immunohistochemical analysis of paraffin-embedded Mouse Spleen Tissue using anti-SMAC/Diablo antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Western Blot: SMAC/Diablo Antibody - BSA Free [NBP1-76772] -

Western Blot: SMAC/Diablo Antibody - BSA Free [NBP1-76772] - Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 u of lysates per lane.Antibodies: SMAC/Diablo 2409 (1 u/mL), SMAC/Diablo (1 u/mL), and beta-actin (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/ Immunofluorescence: SMAC/Diablo Antibody - BSA Free [NBP1-76772] -

Immunocytochemistry/ Immunofluorescence: SMAC/Diablo Antibody - BSA Free [NBP1-76772] - Figure 4 Immunofluorescence Validation of SMAC/Diablo in Mouse Spleen CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Spleen Cells labeling SMAC/Diablo with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).