SMAC/Diablo Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84262

Novus Biologicals, part of Bio-Techne
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NBP1-84262
NBP1-84262-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SMAC/Diablo Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AVYTLTSLYRQYTSLLGKMNSEEEDEVWQVIIGARAEMTSKHQEYLKLETTWMTAVGLSEMAAEAAYQTGADQASITARNHIQLVKLQVEEVHQLSRKAETKLAEAQIEELRQKTQEEG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMAC/Diablo Antibody

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NBP1-84262]

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NBP1-84262]

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NBP1-84262] - Staining in human testis and skeletal muscle tissues using anti-DIABLO antibody. Corresponding DIABLO RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: SMAC/Diablo Antibody [NBP1-84262]

Immunocytochemistry/ Immunofluorescence: SMAC/Diablo Antibody [NBP1-84262]

Immunocytochemistry/Immunofluorescence: SMAC/Diablo Antibody [NBP1-84262] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NBP1-84262]

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NBP1-84262]

Immunohistochemistry-Paraffin: SMAC/Diablo Antibody [NBP1-84262] - Staining of human skeletal muscle shows low expression as expected.
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Applications for SMAC/Diablo Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20-1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMAC/Diablo

Smac/DIABLO is a mitochondrial protein which activates various forms of apoptosis. Specifically, Smac exits the mitochondria and enters the cytosol where it initiates apoptosis by neutralizing members of the IAP family of apoptosis inhibitory proteins. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms.

Long Name

Second Mitochondria-derived Activator of Caspase

Alternate Names

Diablo

Gene Symbol

DIABLO

Additional SMAC/Diablo Products

Product Documents for SMAC/Diablo Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SMAC/Diablo Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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