Sonic Hedgehog/Shh Antibody (171018) [PE] - N-Terminus
Novus Biologicals, part of Bio-Techne | Catalog # IC4641P
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Intracellular Staining by Flow Cytometry
Cited:
Flow Cytometry
Label
PE (Excitation = 488 nm, Emission = 575 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 171018
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
E. coli-derived recombinant mouse Sonic Hedgehog/SHH N-terminus
Cys25-Gly198 (Lys122Arg)
Accession # Q62226
Cys25-Gly198 (Lys122Arg)
Accession # Q62226
Specificity
Detects human and mouse Sonic Hedgehog/SHH in Western blots and mouse Sonic Hedgehog/SHH in ELISAs. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Desert Hedgehog/Dhh (N‑terminus; aa 23-198) is observed and no cross-reactivity with rmIndian Hedgehog/Ihh (N-terminus; aa 66-240), rmDesert Hedgehog/Dhh (C‑terminus; aa 199-396), or rmSonic Hedgehog/Shh (C‑terminus; aa 199-437) is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Sonic Hedgehog/Shh Antibody (171018) [PE] - N-Terminus
Detection of Sonic Hedgehog/Shh in PC‑3 Human Cell Line by Flow Cytometry.
PC-3 human prostate cancer cell line was stained with Rat Anti-Human/Mouse Sonic Hedgehog/Shh N-Terminus PE-conjugated Monoclonal Antibody (Catalog # IC4641P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Sonic Hedgehog/Shh Antibody (171018) [PE] - N-Terminus
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 uL/10^6 cells
Sample: See below
Sample: See below
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Sonic Hedgehog/Shh
Sonic Hedgehog (Shh) is a hedgehog protein that is instrumental in patterning the early embryo. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened.
Additional Sonic Hedgehog/Shh Products
Product Documents for Sonic Hedgehog/Shh Antibody (171018) [PE] - N-Terminus
Product Specific Notices for Sonic Hedgehog/Shh Antibody (171018) [PE] - N-Terminus
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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