Sonic Hedgehog/Shh Antibody (5H4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22126

Novus Biologicals, part of Bio-Techne
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NBP2-22126
NBP2-22126SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Primate

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5H4

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Sonic Hedgehog/Shh Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Sonic Hedgehog (amino acids 26-170) expressed in E. coli. [UniProt# Q15465]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

49.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Sonic Hedgehog/Shh Antibody (5H4)

Western Blot: Sonic Hedgehog/Shh Antibody (5H4) [NBP2-22126]

Western Blot: Sonic Hedgehog/Shh Antibody (5H4) [NBP2-22126]

Western Blot: Sonic Hedgehog/Shh Antibody (5H4) [NBP2-22126] - Analysis using Sonic Hedgehog mouse mAb against LNCaP (1), HepG2 (2), PANC-1 (3),HeLa (4), SK-N-SH (5), F9 (6), NIH3T3 (7), and COS7 (8) cell lysate.
Immunocytochemistry/ Immunofluorescence: Sonic Hedgehog/Shh Antibody (5H4) [NBP2-22126]

Immunocytochemistry/ Immunofluorescence: Sonic Hedgehog/Shh Antibody (5H4) [NBP2-22126]

Immunocytochemistry/Immunofluorescence: Sonic Hedgehog/Shh Antibody (5H4) [NBP2-22126] - Evaluation of IGF-1, IGF1R and HH pathway expression in fibroblasts and oral squamous carcinoma cells. IGF1 mRNA expression in SCC-4 and MF1 cells, evaluated by RT-qPCR. (C) Evaluation of HH pathway component expression in MF1 by immunocytochemistry. Patched 1/PTCH Antibody (NB200-118); Sonic Hedgehog/Shh Antibody (NBP2-22126). Magnification: 400x. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32899449/) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Sonic Hedgehog/Shh Antibody (5H4) [NBP2-22126]

Immunohistochemistry-Paraffin: Sonic Hedgehog/Shh Antibody (5H4) [NBP2-22126]

Immunohistochemistry-Paraffin: Sonic Hedgehog/Shh Antibody (5H4) [NBP2-22126] - Analysis of paraffin-embedded liver cancer tissues using Sonic Hedgehog mouse mAb with DAB staining.
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Applications for Sonic Hedgehog/Shh Antibody (5H4)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 32867229)

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sonic Hedgehog/Shh

This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.

Alternate Names

HHG1, HLP3, HPE3, MCOPCB5, Shh, ShhNC, SMMCI, TPTPS

Entrez Gene IDs

6469 (Human)

Gene Symbol

SHH

UniProt

Q15465

Additional Sonic Hedgehog/Shh Products

Product Documents for Sonic Hedgehog/Shh Antibody (5H4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Sonic Hedgehog/Shh Antibody (5H4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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