Sonic Hedgehog/Shh Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22139

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Sonic Hedgehog/Shh Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an N-terminal portion of the human SHH protein (between residues 1-75) [UniProt Q15465]

Reactivity Notes

Chicken (83%), Zebrafish (84%), Xenopus (81%)

Localization

Cytoplasm. Membrane.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sonic Hedgehog/Shh Antibody - BSA Free

Western Blot: Sonic Hedgehog/Shh AntibodyBSA Free [NBP2-22139]

Western Blot: Sonic Hedgehog/Shh AntibodyBSA Free [NBP2-22139]

Western Blot: Sonic Hedgehog Antibody [NBP2-22139] - WB detection of Sonic Hedgehog in A. human stomach, B. human fetal liver membrane, C. PC12 and D. Cos7
Immunocytochemistry/ Immunofluorescence: Sonic Hedgehog/Shh Antibody - BSA Free [NBP2-22139]

Immunocytochemistry/ Immunofluorescence: Sonic Hedgehog/Shh Antibody - BSA Free [NBP2-22139]

Immunocytochemistry/Immunofluorescence: Sonic Hedgehog Antibody [NBP2-22139] - Sonic Hedgehog antibody was tested in SH-SY5Y cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: Sonic Hedgehog/Shh Antibody - BSA Free [NBP2-22139]

Immunohistochemistry: Sonic Hedgehog/Shh Antibody - BSA Free [NBP2-22139]

Immunohistochemistry: Sonic Hedgehog Antibody [NBP2-22139] - IHC analysis of Sonic Hedgehog in mouse kidney.

Applications for Sonic Hedgehog/Shh Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

1:200 - 1:400

Immunohistochemistry-Paraffin

1:200 - 1:400

Western Blot

1-2 ug/ml
Application Notes
This SHH antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band is observed at ~50 kDa. In Immunocytochemistry/Immunofluorescence cytoplasm/membrane staining was observed in SH-SY5Y cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sonic Hedgehog/Shh

Sonic Hedgehog (SHH), also known as HLP3, HPE3, MCOPCB5, SMMC1, and HHG1, is a gene that encodes for a protein instrumental in patterning the early embryo. Specifically, SHH has been implicated as the main signal in mapping out the development of the ventral neural tube, the anterior-posterior limb access, and the ventral somites. This occurs by SHH binding to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. SHH also regulates another target gene, the gli oncogene. In adults, SHH helps to control stem cell proliferation. The N-terminal form of SHH is associated with lipid rafts at the cell surface, while on a tissue level SHH is expressed in fetal intestine, liver, lung and kidney. Recent research has found SHH to modulate the spatial organization of neuroepithelial cell proliferation in the embryonic chick (PMID: 23031710), as well as detected a role for SHH in Idiopathic Pulmonary Fibrosis (PMID: 23023967).

Alternate Names

HHG1, HLP3, HPE3, MCOPCB5, Shh, ShhNC, SMMCI, TPTPS

Entrez Gene IDs

6469 (Human); 20423 (Mouse); 29499 (Rat)

Gene Symbol

SHH

UniProt

Q62226, Q15465, Q63673 (Rat)

Additional Sonic Hedgehog/Shh Products

Product Documents for Sonic Hedgehog/Shh Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Sonic Hedgehog/Shh Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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