SSEA-1 Antibody (MEM-158)

Novus Biologicals, part of Bio-Techne | Catalog # NB500-376

Novus Biologicals, part of Bio-Techne
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NB500-376
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Key Product Details

Species Reactivity

Validated:

Human, Bovine (Negative), Porcine (Negative), Sheep (Negative)

Applications

CyTOF-ready, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # MEM-158

Concentration

1 mg/ml

View all SSEA-1 Antibodies »

Product Summary for SSEA-1 Antibody (MEM-158)

Immunogen

Human granulocytes

Localization

Cell Membrane

Specificity

The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. HLDA VI; WS Code AS A053

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for SSEA-1 Antibody (MEM-158)

Flow Cytometry: SSEA-1 Antibody (MEM-158) [NB500-376]

Flow Cytometry: SSEA-1 Antibody (MEM-158) [NB500-376]

Flow Cytometry: CD15/Lewis X Antibody (MEM-158) [NB500-376] - Analysis using the FITC conjugate of NB500-376. Staining of human peripheral blood cells with anti-human CD15 (MEM-158) FITC. Cells in the granulocyte gate were used for analysis.
Flow Cytometry: SSEA-1 Antibody (MEM-158) [NB500-376]

Flow Cytometry: SSEA-1 Antibody (MEM-158) [NB500-376]

Flow Cytometry: CD15/Lewis X Antibody (MEM-158) [NB500-376] - Surface staining (mass cytometry) of human peripheral blood cells (after ammonium chloride red blood cell lysis) with anti-human CD15 (MEM-158) 173Yb. Gated on singlets.

Applications for SSEA-1 Antibody (MEM-158)

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunoprecipitation

1:50
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

TBS (pH 8.0)

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SSEA-1

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

Long Name

Stage-specific Embryonic Antigen-1

Alternate Names

SSEA1

Gene Symbol

FUT4

Additional SSEA-1 Products

Product Documents for SSEA-1 Antibody (MEM-158)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SSEA-1 Antibody (MEM-158)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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