SSEA-1 Antibody (MEM-158) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB500-377

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB500-377
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Bovine (Negative), Porcine (Negative), Sheep (Negative)

Applications

Flow Cytometry, Immunoprecipitation

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgM Clone # MEM-158

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SSEA-1 Antibodies »

Product Summary for SSEA-1 Antibody (MEM-158) [FITC]

Immunogen

Human granulocytes

Localization

Cell Membrane

Specificity

The antibody MEM-158 reacts with CD15, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells. HLDA VI; WS Code AS A053

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for SSEA-1 Antibody (MEM-158) [FITC]

Flow Cytometry: CD15/Lewis X Antibody (MEM-158) [FITC] [NB500-377] - Fig. 1. Surface staining of human peripheral blood cells with anti-human CD15 (MEM-158) FITC. Cells in the granulocyte gate were used for analysis.

Applications for SSEA-1 Antibody (MEM-158) [FITC]

Application
Recommended Usage

Flow Cytometry

20 ul/100 ul of whole blood or 10^6 cells in suspension

Immunoprecipitation

1:10-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

TBS and 0.2% (w/v) high grade BSA (protease free)

Preservative

0.9% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: SSEA-1

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.

Long Name

Stage-specific Embryonic Antigen-1

Alternate Names

SSEA1

Gene Symbol

FUT4

Additional SSEA-1 Products

Product Documents for SSEA-1 Antibody (MEM-158) [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SSEA-1 Antibody (MEM-158) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.