SURF5 Antibody (PCRP-MED22-1E4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13787

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2C Clone # PCRP-MED22-1E4

Concentration

0.2 mg/ml

View all SURF5 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human SURF5 protein (Uniprot: Q15528)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2C

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14190)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for SURF5 Antibody (PCRP-MED22-1E4)

Immunocytochemistry/ Immunofluorescence: SURF5 Antibody (PCRP-MED22-1E4) [NBP3-13787]

Immunocytochemistry/ Immunofluorescence: SURF5 Antibody (PCRP-MED22-1E4) [NBP3-13787]

Immunocytochemistry/Immunofluorescence: SURF5 Antibody (PCRP-MED22-1E4) [NBP3-13787] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SURF5 Antibody (PCRP-MED22-1E4) followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.
Flow Cytometry: SURF5 Antibody (PCRP-MED22-1E4) [NBP3-13787]

Flow Cytometry: SURF5 Antibody (PCRP-MED22-1E4) [NBP3-13787]

Flow Cytometry: SURF5 Antibody (PCRP-MED22-1E4) [NBP3-13787] - Flow cytometric analysis of PFA-fixed HeLa cells. SURF5 antibody (PCRP-MED22-1E4) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Protein Array: SURF5 Antibody (PCRP-MED22-1E4) [NBP3-13787]

Protein Array: SURF5 Antibody (PCRP-MED22-1E4) [NBP3-13787]

Protein Array: SURF5 Antibody (PCRP-MED22-1E4) [NBP3-13787] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SURF5 Antibody (PCRP-MED22-1E4).

Applications for SURF5 Antibody (PCRP-MED22-1E4)

Application
Recommended Usage

Flow Cytometry

0.5-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SURF5

SURF5 is located in the surfeit gene cluster, a group of very tightly linked housekeeping genes that do not share sequence similarity. The gene is oriented in a head-to-head fashion with RPL7A (SURF3) and the two genes share a bidirectional promoter. The encoded proteins are localized to the cytoplasm. Two alternative transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]

Alternate Names

Med24, mediator complex subunit 22SURF5surfeit 5, mediator of RNA polymerase II transcription subunit 22, MGC48682, Surf-5, Surfeit locus protein 5

Gene Symbol

MED22

UniProt

Q15528 (Human)

Additional SURF5 Products

Product Documents for SURF5 Antibody (PCRP-MED22-1E4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SURF5 Antibody (PCRP-MED22-1E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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