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SURF5 Antibody (PCRP-MED22-1E4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14190

Novus Biologicals, part of Bio-Techne
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NBP3-14190

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2C Clone # PCRP-MED22-1E4

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant full-length human SURF5 protein (Uniprot: Q15528)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2C

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13787).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for SURF5 Antibody (PCRP-MED22-1E4) - Azide and BSA Free

Protein Array: SURF5 Antibody (PCRP-MED22-1E4) - Azide and BSA Free [NBP3-14190]

Protein Array: SURF5 Antibody (PCRP-MED22-1E4) - Azide and BSA Free [NBP3-14190]

Protein Array: SURF5 Antibody (PCRP-MED22-1E4) - Azide and BSA Free [NBP3-14190] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SURF5 Antibody (PCRP-MED22-1E4).

Applications for SURF5 Antibody (PCRP-MED22-1E4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-2 ug/ml

Immunoprecipitation

1-2 ug per 100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order this antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: SURF5

SURF5 is located in the surfeit gene cluster, a group of very tightly linked housekeeping genes that do not share sequence similarity. The gene is oriented in a head-to-head fashion with RPL7A (SURF3) and the two genes share a bidirectional promoter. The encoded proteins are localized to the cytoplasm. Two alternative transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]

Alternate Names

Med24, mediator complex subunit 22SURF5surfeit 5, mediator of RNA polymerase II transcription subunit 22, MGC48682, Surf-5, Surfeit locus protein 5

Gene Symbol

MED22

Additional SURF5 Products

Product Documents for SURF5 Antibody (PCRP-MED22-1E4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SURF5 Antibody (PCRP-MED22-1E4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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