Survivin Antibody (BIRC5/8936R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23790

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-23790-100ug
NBP3-23790-20ug
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # BIRC5/8936R

Concentration

0.2 mg/ml

View all Survivin Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa1-100) of human Survivin protein (exact sequence is proprietary)

Localization

Nucleus. Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Store at 2 to 8C. Non-hazardous. No MSDS required.

Scientific Data Images for Survivin Antibody (BIRC5/8936R)

Survivin Antibody (BIRC5/8936R) Immunohistochemistry-Paraffin: Survivin Antibody (BIRC5/8936R) [NBP3-23790] -

Immunohistochemistry-Paraffin: Survivin Antibody (BIRC5/8936R) [NBP3-23790] -

Immunohistochemistry-Paraffin: Survivin Antibody (BIRC5/8936R) [NBP3-23790] - Formalin-fixed, paraffin-embedded human ovarian cancer stained with Survivin Antibody (BIRC5/8936R). Inset: PBS instead of primary antibody; secondary only negative control.
Survivin Antibody (BIRC5/8936R) Immunohistochemistry-Paraffin: Survivin Antibody (BIRC5/8936R) [NBP3-23790] -

Immunohistochemistry-Paraffin: Survivin Antibody (BIRC5/8936R) [NBP3-23790] -

Immunohistochemistry-Paraffin: Survivin Antibody (BIRC5/8936R) [NBP3-23790] - Formalin-fixed, paraffin-embedded human testis stained with Survivin Antibody (BIRC5/8936R). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for Survivin Antibody (BIRC5/8936R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Survivin

Survivin (BIRC5 or IAP4) is the smallest member of the inhibitor of apoptosis (IAP) proteins, containing only a single baculovirus IAP repeat (BIR) domain and lacking the C-terminal RING domain. Survivin has a theoretical molecular weight of 16.5 kDa (wild-type protein) and exists as ten splice variant forms including the most predominant isoforms, survivin-2B and surviving-DeltaEx3 (1). It also undergoes phosphorylation by PKA, Plk1, Cdk1, CKII and Aurora B at multiple sites (e.g. Serine 20 and Threonine 34, 53 and 117).

Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).

Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).

References

1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993

2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829

3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431

Alternate Names

API4, BIRC5

Gene Symbol

BIRC5

Additional Survivin Products

Product Documents for Survivin Antibody (BIRC5/8936R)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Survivin Antibody (BIRC5/8936R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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