Survivin Antibody (BIRC5/8936R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24238
Recombinant Monoclonal Antibody
![Survivin Antibody (BIRC5/8936R) - Azide and BSA Free Immunohistochemistry-Paraffin: Survivin Antibody (BIRC5/8936R) - Azide and BSA Free [NBP3-24238] -](https://resources.bio-techne.com/images/products/nbp3-24238_rabbit-survivin-mab-birc5-8936r-azide-and-bsa-free-24120257592860.jpg "Immunohistochemistry-Paraffin: Survivin Antibody (BIRC5/8936R) - Azide and BSA Free [NBP3-24238] -")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Kappa Clone # BIRC5/8936R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa1-100) of human Survivin protein (exact sequence is proprietary)
Localization
Nucleus. Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Antibody without azide - store at -20 to -80C.
Scientific Data Images for Survivin Antibody (BIRC5/8936R) - Azide and BSA Free
Immunohistochemistry-Paraffin: Survivin Antibody (BIRC5/8936R) - Azide and BSA Free [NBP3-24238] -
Immunohistochemistry-Paraffin: Survivin Antibody (BIRC5/8936R) - Azide and BSA Free [NBP3-24238] - Formalin-fixed, paraffin-embedded human testis stained with Survivin Antibody (BIRC5/8936R). Inset: PBS instead of primary antibody; secondary only negative control.![Survivin Antibody (BIRC5/8936R) - Azide and BSA Free Immunohistochemistry-Paraffin: Survivin Antibody (BIRC5/8936R) - Azide and BSA Free [NBP3-24238] -](https://resources.bio-techne.com/images/products/nbp3-24238_rabbit-survivin-mab-birc5-8936r-azide-and-bsa-free-24120257595042.jpg "Immunohistochemistry-Paraffin: Survivin Antibody (BIRC5/8936R) - Azide and BSA Free [NBP3-24238] -")
Immunohistochemistry-Paraffin: Survivin Antibody (BIRC5/8936R) - Azide and BSA Free [NBP3-24238] -
Immunohistochemistry-Paraffin: Survivin Antibody (BIRC5/8936R) - Azide and BSA Free [NBP3-24238] - Formalin-fixed, paraffin-embedded human ovarian cancer stained with Survivin Antibody (BIRC5/8936R). Inset: PBS instead of primary antibody; secondary only negative control.Applications for Survivin Antibody (BIRC5/8936R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Survivin
Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).
Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).
References
1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993
2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829
3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431
Alternate Names
API4, BIRC5
Gene Symbol
BIRC5
Additional Survivin Products
Product Documents for Survivin Antibody (BIRC5/8936R) - Azide and BSA Free
Product Specific Notices for Survivin Antibody (BIRC5/8936R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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