Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7H12

Format

BSA Free

Concentration

1.0 mg/ml

View all Synaptophysin Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Synaptophysin expressed in E. coli. [UniProt# P08247]

Marker

Pre-synaptic marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

33.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Synaptophysin Antibody (7H12) - BSA Free

Western Blot: Synaptophysin Antibody (7H12)BSA Free [NBP1-47483]

Western Blot: Synaptophysin Antibody (7H12)BSA Free [NBP1-47483]

Western Blot: Synaptophysin Antibody (7H12) [NBP1-47483] - Analysis using Synaptophysin mouse mAb against rat brain tissue lysate.
Immunohistochemistry-Paraffin: Synaptophysin Antibody (7H12) - BSA Free [NBP1-47483]

Immunohistochemistry-Paraffin: Synaptophysin Antibody (7H12) - BSA Free [NBP1-47483]

Immunohistochemistry-Paraffin: Synaptophysin Antibody (7H12) [NBP1-47483] - Human pancreas (FFPE) stained with Synaptophysin antibody. pH 9 HIER antigen retrieval. Image from verified customer review.
Immunohistochemistry-Paraffin: Synaptophysin Antibody (7H12) - BSA Free [NBP1-47483]

Immunohistochemistry-Paraffin: Synaptophysin Antibody (7H12) - BSA Free [NBP1-47483]

Immunohistochemistry-Paraffin: Synaptophysin Antibody (7H12) [NBP1-47483] - Analysis of paraffin-embedded human small intestine, myenteric plexus tissues using anti-Synaptophysin mouse mAb.

Applications for Synaptophysin Antibody (7H12) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Reviewed Applications

Read 1 review rated 5 using NBP1-47483 in the following applications:

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Synaptophysin

Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore. Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases. Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex.

Alternate Names

SYP

Entrez Gene IDs

6855 (Human)

Gene Symbol

SYP

UniProt

P08247

Additional Synaptophysin Products

Product Documents for Synaptophysin Antibody (7H12) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Synaptophysin Antibody (7H12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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