Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7H12

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Synaptophysin Antibodies »

Product Summary for Synaptophysin Antibody (7H12) - Azide and BSA Free

Immunogen

Purified recombinant fragment of human Synaptophysin expressed in E. coli. [UniProt# P08247]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

33.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Synaptophysin Antibody (7H12) - Azide and BSA Free

Western Blot: Synaptophysin Antibody (7H12)Azide and BSA Free [NBP2-80972]

Western Blot: Synaptophysin Antibody (7H12)Azide and BSA Free [NBP2-80972]

Western Blot: Synaptophysin Antibody (7H12) - Azide and BSA Free [NBP2-80972] - Analysis using Synaptophysin mouse mAb against rat brain tissue lysate. Image from the standard format of this antibody.
Immunohistochemistry: Synaptophysin Antibody (7H12) - Azide and BSA Free [NBP2-80972]

Immunohistochemistry: Synaptophysin Antibody (7H12) - Azide and BSA Free [NBP2-80972]

Immunohistochemistry: Synaptophysin Antibody (7H12) - Azide and BSA Free [NBP2-80972] - Analysis of paraffin-embedded human small intestine, myenteric plexus tissues using anti-Synaptophysin mouse mAb. Image from the standard format of this antibody.
Immunohistochemistry: Synaptophysin Antibody (7H12) - Azide and BSA Free [NBP2-80972]

Immunohistochemistry: Synaptophysin Antibody (7H12) - Azide and BSA Free [NBP2-80972]

Immunohistochemistry: Synaptophysin Antibody (7H12) - Azide and BSA Free [NBP2-80972] - Human pancreas (FFPE) stained with Synaptophysin antibody. pH 9 HIER antigen retrieval. Image from verified customer review. Image from the standard format of this antibody.

Applications for Synaptophysin Antibody (7H12) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Synaptophysin

Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells.Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore. Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases. Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex.

Alternate Names

SYP

Gene Symbol

SYP

Additional Synaptophysin Products

Product Documents for Synaptophysin Antibody (7H12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Synaptophysin Antibody (7H12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.