Synaptophysin Antibody (SYP/7976R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20528
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # SYP/7976R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 274-313) of human Synaptophysin protein (exact sequence is proprietary)
Localization
Cytoplasm.
Specificity
This recombinant rabbit monoclonal antibody recognizes a protein of 38kDa that is identified as synaptophysin It is an N-glycosylated integral membrane protein found in neurons and endocrine cells Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: HepG2, HeLa or Y79 cells. Human pancreas, cerebellum or pheochromocytoma.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for Synaptophysin Antibody (SYP/7976R)
Synaptophysin Antibody (SYP/7976R)
Formalin-fixed, paraffin-embedded human adrenal stained with Synaptophysin antibody (SYP/7976R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for Synaptophysin Antibody (SYP/7976R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Synaptophysin
Alternate Names
SYP
Gene Symbol
SYP
Additional Synaptophysin Products
Product Documents for Synaptophysin Antibody (SYP/7976R)
Product Specific Notices for Synaptophysin Antibody (SYP/7976R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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