T-bet/TBX21 Antibody (4B10) [mFluor Violet 450 SE]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43298MFV450

Novus Biologicals, part of Bio-Techne
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NBP1-43298
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Key Product Details

Species Reactivity

Human, Mouse, Rhesus Macaque

Applications

Flow (Intracellular), Flow Cytometry, Immunoprecipitation, Western Blot

Label

mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4B10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all T-bet/TBX21 Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was TBet.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for T-bet/TBX21 Antibody (4B10) [mFluor Violet 450 SE]

T-bet/TBX21 Antibody (4B10) [mFluor Violet 450 SE] [NBP1-43298MFV450] -

T-bet/TBX21 Antibody (4B10) [mFluor Violet 450 SE] [NBP1-43298MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.

Applications for T-bet/TBX21 Antibody (4B10) [mFluor Violet 450 SE]

Application
Recommended Usage

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: T-bet/TBX21

Tbx21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq]

Long Name

T-box transcription factor TBX21

Alternate Names

T-PET, Tbet, TBLYM, TBX21

Gene Symbol

TBX21

Additional T-bet/TBX21 Products

Product Documents for T-bet/TBX21 Antibody (4B10) [mFluor Violet 450 SE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for T-bet/TBX21 Antibody (4B10) [mFluor Violet 450 SE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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