TfR (Transferrin R) Antibody (MEM-189)

Novus Biologicals, part of Bio-Techne | Catalog # NB500-493

Clone MEM-189 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB500-493-0.1mg
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunoprecipitation, Western Blot

Cited:

In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-189

Concentration

1.0 mg/ml

View all TfR (Transferrin R) Antibodies »

Product Specifications

Immunogen

KG1 human acute myelogenous leukaemia cell line (NM_001128148.1).

Localization

Cell membrane; Single-pass type II membrane protein. Melanosome (identified by mass spectrometry in melanosome fractions from stage I to stage IV). Transferrin receptor protein 1, serum form: Secreted.

Specificity

The antibody MEM-189 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor. HLDA VI; WS Code NL N-L025

Marker

Recycling Endosome Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TfR (Transferrin R) Antibody (MEM-189)

Flow Cytometry: TfR (Transferrin R) Antibody (MEM-189) [NB500-493]

Flow Cytometry: TfR (Transferrin R) Antibody (MEM-189) [NB500-493]

Flow Cytometry: TfR (Transferrin R) Antibody (MEM-189) [NB500-493] - taining of CD71 in K562 cells with anti-CD71 (MEM-189) purified, GAM-APC.

Applications for TfR (Transferrin R) Antibody (MEM-189)

Application
Recommended Usage

Flow Cytometry

2 ug/ml

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
This antibody is useful for Western Blot analysis (non-reducing), immunoprecipitation, and flow cytometry. Purity is > 95% (by SDS-PAGE). This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH 8.0)

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TfR (Transferrin R)

CD71, also known as the transferrin receptor (TFR), is a type II membrane glycoprotein that exists as a disulfide-linked homodimer of two identical subunits. CD71 binds to two molecules of transferrin and a serum irontransport protein, and directs the cellular uptake of iron via receptor-mediated endocytosis. CD71 is expressed, typically at high levels, on all proliferating cells, reticulocytes and erythroid precursors. It is not expressed on resting leukocytes, but is upregulated upon activation of lymphocytes, monocytes and macrophages. CD71 is also found on most dividing cells and on brain endothelium. A second transferrin receptor, TFR2, also mediates the uptake of transferrin-bound iron. TFR2 is a two-subunit homodimer and is highly expressed in liver as well as in hepatocytes and erythroid precursors. Mutations in the TFR2 gene result in hereditary hemochromatosis type III (HFE3), an iron overloading disorder predominant in Caucasians.

Long Name

Transferrin Receptor

Alternate Names

CD71, TfR (TransferrinR), TFR1, TFRC, TRFR

Entrez Gene IDs

7037 (Human)

Gene Symbol

TFRC

UniProt

P02786 (Human)

Additional TfR (Transferrin R) Products

Product Documents for TfR (Transferrin R) Antibody (MEM-189)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TfR (Transferrin R) Antibody (MEM-189)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.