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TfR (Transferrin R) Antibody (MEM-189) [PE/Cy5.5]

Novus Biologicals, part of Bio-Techne | Catalog # NB500-493PECY55

Clone MEM-189 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB500-493PECY55

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

PE/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-189

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

KG1 human acute myelogenous leukaemia cell line (NM_001128148.1).

Localization

Cell membrane; Single-pass type II membrane protein. Melanosome (identified by mass spectrometry in melanosome fractions from stage I to stage IV). Transferrin receptor protein 1, serum form: Secreted.

Specificity

The antibody MEM-189 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor. HLDA VI; WS Code NL N-L025

Marker

Recycling Endosome Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for TfR (Transferrin R) Antibody (MEM-189) [PE/Cy5.5]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: TfR (Transferrin R)

Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity.

Long Name

Transferrin Receptor

Alternate Names

CD71, TfR (TransferrinR), TFR1, TFRC, TRFR

Gene Symbol

TFRC

Additional TfR (Transferrin R) Products

Product Documents for TfR (Transferrin R) Antibody (MEM-189) [PE/Cy5.5]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TfR (Transferrin R) Antibody (MEM-189) [PE/Cy5.5]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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