Thyroid Peroxidase Antibody (TPO/1922)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79789

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # TPO/1922

Concentration

0.2 mg/ml

View all Thyroid Peroxidase Antibodies »

Product Summary for Thyroid Peroxidase Antibody (TPO/1922)

Immunogen

Recombinant fragment of human Thyroid Peroxidase (around aa 685-804) (Exact sequence is proprietary) (Uniprot: P07202)

Localization

Cytoplasmic, Cell Surface

Specificity

Thyroperoxidase (TPO) is a 933-amino acid, type I transmembrane glycoprotein that plays a key role in thyroid gland function and autoimmunity. It is present as a dimer on the apical surface of thyroid follicular cells. TPO functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Malignant thyroid tumors exhibit an anomaly in TPO resulting in lower affinity for anti-TPO. This antibody may aid in the differentiation between benign and malignant thyroid tumors.

Marker

Thyroid Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79923)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Thyroid Peroxidase Antibody (TPO/1922)

Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody (TPO/1922) [NBP2-79789]

Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody (TPO/1922) [NBP2-79789]

Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody (TPO/1922) [NBP2-79789] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with Thyroid Peroxidase Antibody (TPO/1922).
Protein Array: Thyroid Peroxidase Antibody (TPO/1922) [NBP2-79789]

Protein Array: Thyroid Peroxidase Antibody (TPO/1922) [NBP2-79789]

Protein Array: Thyroid Peroxidase Antibody (TPO/1922) [NBP2-79789] - Analysis of Protein Array containing >19,000 full-length human proteins using Thyroid Peroxidase Antibody (TPO/1922) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5

Applications for Thyroid Peroxidase Antibody (TPO/1922)

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Thyroid Peroxidase

Thyroid Peroxidase encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Additional splice variants have been described but their biological natures have not been determined. [provided by RefSeq]

Alternate Names

EC 1.11.1, MSA, TDH2A, thyroid microsomal antigen, thyroid peroxidase, thyroperoxidase, TPXEC 1.11.1.8

Gene Symbol

TPO

UniProt

P07202 (Human)

Additional Thyroid Peroxidase Products

Product Documents for Thyroid Peroxidase Antibody (TPO/1922)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Thyroid Peroxidase Antibody (TPO/1922)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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