Thyroid Peroxidase Antibody (TPO/1922) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79923
![Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody (TPO/1922) - Azide and BSA Free [NBP2-79923]](https://resources.bio-techne.com/images/products/Thyroid-Peroxidase-Antibody-TPO-1922-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79923-img0001.jpg "Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody (TPO/1922) - Azide and BSA Free [NBP2-79923]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # TPO/1922
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Thyroid Peroxidase Antibody (TPO/1922) - Azide and BSA Free
Immunogen
Recombinant fragment of human Thyroid Peroxidase (around aa 685-804) (Exact sequence is proprietary) (Uniprot: P07202)
Localization
Cytoplasmic, Cell Surface
Specificity
Thyroperoxidase (TPO) is a 933-amino acid, type I transmembrane glycoprotein that plays a key role in thyroid gland function and autoimmunity. It is present as a dimer on the apical surface of thyroid follicular cells. TPO functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Malignant thyroid tumors exhibit an anomaly in TPO resulting in lower affinity for anti-TPO. This antibody may aid in the differentiation between benign and malignant thyroid tumors.
Marker
Thyroid Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79789).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Thyroid Peroxidase Antibody (TPO/1922) - Azide and BSA Free
Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody (TPO/1922) - Azide and BSA Free [NBP2-79923]
Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody (TPO/1922) - Azide and BSA Free [NBP2-79923] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with Thyroid Peroxidase Mouse Monoclonal Antibody (TPO/1922).![Protein Array: Thyroid Peroxidase Antibody (TPO/1922) - Azide and BSA Free [NBP2-79923]](https://resources.bio-techne.com/images/products/Thyroid-Peroxidase-Antibody-TPO-1922-Azide-and-BSA-Free-Protein-Array-NBP2-79923-img0002.jpg "Protein Array: Thyroid Peroxidase Antibody (TPO/1922) - Azide and BSA Free [NBP2-79923]")
Protein Array: Thyroid Peroxidase Antibody (TPO/1922) - Azide and BSA Free [NBP2-79923]
Protein Array: Thyroid Peroxidase Antibody (TPO/1922) - Azide and BSA Free [NBP2-79923] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (mAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAbto protein X is equal to 29.Applications for Thyroid Peroxidase Antibody (TPO/1922) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Thyroid Peroxidase
Alternate Names
EC 1.11.1, MSA, TDH2A, thyroid microsomal antigen, thyroid peroxidase, thyroperoxidase, TPXEC 1.11.1.8
Gene Symbol
TPO
Additional Thyroid Peroxidase Products
Product Documents for Thyroid Peroxidase Antibody (TPO/1922) - Azide and BSA Free
Product Specific Notices for Thyroid Peroxidase Antibody (TPO/1922) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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