TIM-1/KIM-1/HAVCR Antibody (JE39-41)

Western Blot: TIM-1/KIM-1/HAVCR Antibody (JE39-41) [NBP3-33017]

Western Blot: TIM-1/KIM-1/HAVCR Antibody (JE39-41) [NBP3-33017] - Western blot analysis of TIM-1/KIM-1/HAVCR on different lysates with Rabbit anti-TIM-1/KIM-1/HAVCR antibody (NBP3-33017) at 1/1,000 dilution.

Lane 1: Huh7 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: NCI-H441 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: RAW264.7 cell lysate
Lane 7: PC-12 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 39 kDa
Observed band size: 50 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33017) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.