Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow (Cell Surface), Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # F38-2E2

Format

Azide Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TIM-3 Antibodies »

Product Summary for TIM-3 Antibody (F38-2E2) - Azide Free

Immunogen

This antibody was raised against recombinant human TIM-3. [Uniprot# Q8TDQ0]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TIM-3 Antibody (F38-2E2) - Azide Free

Flow Cytometry: TIM-3 Antibody (F38-2E2) - Azide Free [NBP2-27221]

Flow Cytometry: TIM-3 Antibody (F38-2E2) - Azide Free [NBP2-27221]

Flow Cytometry: TIM-3 Antibody (F38-2E2) - Azide Free [NBP2-27221] - TIM-3 Antibody (F38-2E2) [Azide Free] [NBP2-27221] - Cell surface staining of unstimulated (A) and Con A stimulated (B) human PBMCs with 0.6 ug of purified human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)and PE-conjugated secondary antibody this antibody was used for this test (cells were not fixed for testing).
Flow Cytometry: TIM-3 Antibody (F38-2E2) - Azide Free [NBP2-27221]

Flow Cytometry: TIM-3 Antibody (F38-2E2) - Azide Free [NBP2-27221]

Flow Cytometry: TIM-3 Antibody (F38-2E2) - Azide Free [NBP2-27221] - Analysis using the PE conjugate of NBP2-27220. Staining of Con A stimulated human PBMCs with PE-conjugated human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)was used for this test (cells were not fixed for testing).
Flow (Cell Surface): TIM-3 Antibody (F38-2E2) - Azide Free [NBP2-27221]

Flow (Cell Surface): TIM-3 Antibody (F38-2E2) - Azide Free [NBP2-27221]

Flow (Cell Surface): TIM-3 Antibody (F38-2E2) - Azide Free [NBP2-27221] - TIM-3 Antibody (F38-2E2) - (Azide Free) [NBP2-27221] - hPBMC cells were either stimulated with Con A (panel B) or grown in normal media (panel A). A cell surface stain was performed with TIM-3 (F38-2E2) NBP2-27221 and Mouse IgG1 Kappa NBP2-27287. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes. A co-stain was also performed with CD4 (RPA-T4) NBP2-27216AF647.

Applications for TIM-3 Antibody (F38-2E2) - Azide Free

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow (Cell Surface)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Filter sterilized PBS

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TIM-3

T cell immunoglobulin and mucin-domain containing 3 (TIM-3) is a type I transmembrane protein that functions in suppressing immune cell responses and is considered a checkpoint receptor (1,2). TIM-3 is an inhibitory receptor that was first identified as a cell marker for interferon-gamma (IFN-gamma)-producing CD4+ T helper (Th1) and CD8+ T cytotoxic (Tc1) cells (1,2). TIM-3 is also expressed on other immune cell types: regulatory T cells (Treg), natural killer (NK) cells, macrophages, and dendritic cells (DCs), as well as leukemia stem cells (LSCs) (1-3). Human TIM-3 protein is 301 amino acids (aa) in length with a theoretical molecular weight (MW) of 33.4 kDa and shares ~63% aa sequence identity with mouse TIM-3 (2,4). The TIM-3 protein has an extracellular IgV domain, a mucin and stalk domain with O- and N-glycosylation sites, a transmembrane domain, and an intracellular tail with conserved tyrosine residues (2,3). Ligands for TIM-3 include soluble galectin-9, high-mobility group protein B1 (HMGB1), phosphatidylserine (PtdSer), and carcinoembyronic antigen-related cell adhesion molecule-1 (CEACAM-1) (1-3,5). Each of these ligands interact with different regions of the TIM-3 IgV domain. In the absence of ligand binding, the unphosphorylated intracellular tyrosine residues of TIM-3 are associated with HLA-B associated transcript 3 (Bat3), which recruits Lck and this Bat3-Lck complex preserves T cell signaling (1-3,5). When TIM-3 is engaged, an intracellular signaling cascade is initiated to inhibit immune cell activation. (1-3,5). Specifically, the tyrosine residues of TIM-3 are phosphorylated, Bat3 is released, and this results in suppression of immune responses (1-3, 5). Persistent, sustained TIM-3 signaling eventually results in T cell exhaustion (1-3,5).

Dysregulation of TIM-3 expression is associated with autoimmune diseases as shown by studies where inhibition of TIM-3 using blocking antibodies worsened disease progression in experimental autoimmune encephalomyelitis (EAE) models of multiple sclerosis (MS) (2,3,5). Conversely, high levels of TIM-3 have been shown during viral infection as well as in many cancer types where its increased expression may be an indicator of poor prognosis (2,3,5). TIM-3 has emerged as a potential cancer immunotherapy target as preclinical studies blocking TIM-3 results in increased anti-tumor immunity and prevents tumor growth (3,5). Studies have suggested combination therapy of TIM-3 blockade with blockade of other checkpoint inhibitors such as programmed death 1 (PD-1) or lymphocyte activation gene 3 (LAG-3) is more effective than TIM-3 blockade alone (3,5).

References

1. Acharya N, Sabatos-Peyton C, Anderson AC. Tim-3 finds its place in the cancer immunotherapy landscape. J Immunother Cancer. 2020; 8(1):e000911. https://doi.org/10.1136/jitc-2020-000911

2. Das M, Zhu C, Kuchroo VK. Tim-3 and its role in regulating anti-tumor immunity. Immunol Rev. 2017; 276(1):97-111. https://doi.org/10.1111/imr.12520

3. Joller N, Kuchroo VK. Tim-3, Lag-3, and TIGIT. Curr Top Microbiol Immunol. 2017; 410:127-156. https://doi.org/10.1007/82_2017_62

4. Uniprot (Q8TDQ0)

5. Wolf Y, Anderson AC, Kuchroo VK. TIM3 comes of age as an inhibitory receptor. Nat Rev Immunol. 2020; 20(3):173-185. https://doi.org/10.1038/s41577-019-0224-6

Long Name

T Cell Immunoglobulin Mucin-3

Alternate Names

CD366, HAVcr-2, HAVCR2, KIM-3, SPTCL, TIM3, TIMD3

Gene Symbol

HAVCR2

Additional TIM-3 Products

Product Documents for TIM-3 Antibody (F38-2E2) - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TIM-3 Antibody (F38-2E2) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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