TIMP-2 Antibody (OTI1A6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74521

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all TIMP-2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TIMP2(NP_003246) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TIMP-2 Antibody (OTI1A6) - Azide and BSA Free

Western Blot: TIMP-2 Antibody (OTI1A6)Azide and BSA Free [NBP2-74521]

Western Blot: TIMP-2 Antibody (OTI1A6)Azide and BSA Free [NBP2-74521]

Western Blot: TIMP-2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74521] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIMP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIMP2.
Immunohistochemistry: TIMP-2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74521]

Immunohistochemistry: TIMP-2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74521]

Immunohistochemistry: TIMP-2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74521] - Staining of paraffin-embedded Human endometrium tissue using anti-TIMP2 mouse monoclonal antibody.
Flow Cytometry: TIMP-2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74521]

Flow Cytometry: TIMP-2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74521]

Flow Cytometry: TIMP-2 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74521] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-TIMP2 antibody, and then analyzed by flow cytometry.
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Applications for TIMP-2 Antibody (OTI1A6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TIMP-2

The TIMP2 gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role a

Long Name

Tissue Inhibitors of Metalloproteinases 2

Alternate Names

TIMP2

Gene Symbol

TIMP2

Additional TIMP-2 Products

Product Documents for TIMP-2 Antibody (OTI1A6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TIMP-2 Antibody (OTI1A6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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