TIMP-2 Antibody (TIMP2/2044)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07720
![Immunohistochemistry-Paraffin: TIMP-2 Antibody (TIMP2/2044) [NBP3-07720]](https://resources.bio-techne.com/images/products/TIMP-2-Antibody-TIMP2-2044-Immunohistochemistry-Paraffin-NBP3-07720-img0002.jpg "Immunohistochemistry-Paraffin: TIMP-2 Antibody (TIMP2/2044) [NBP3-07720]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # TIMP2/2044
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human TIMP-2 protein fragment (aa 48-220) (exact sequence is proprietary) (Uniprot: P16035)
Localization
Cytoplasm and Cell Surface
Specificity
It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08714)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for TIMP-2 Antibody (TIMP2/2044)
Immunohistochemistry-Paraffin: TIMP-2 Antibody (TIMP2/2044) [NBP3-07720]
Immunohistochemistry-Paraffin: TIMP-2 Antibody (TIMP2/2044) [NBP3-07720] - Formalin-fixed, paraffin-embedded human Placenta stained with TIMP-2 Mouse Monoclonal Antibody (TIMP-2/2044).![Protein Array: TIMP-2 Antibody (TIMP2/2044) [NBP3-07720]](https://resources.bio-techne.com/images/products/TIMP-2-Antibody-TIMP2-2044-Protein-Array-NBP3-07720-img0001.jpg "Protein Array: TIMP-2 Antibody (TIMP2/2044) [NBP3-07720]")
Protein Array: TIMP-2 Antibody (TIMP2/2044) [NBP3-07720]
Protein Array: TIMP-2 Antibody (TIMP2/2044) [NBP3-07720] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TIMP-2 Mouse Monoclonal Antibody (TIMP-2/2044).Applications for TIMP-2 Antibody (TIMP2/2044)
Application
Recommended Usage
ELISA
2-4 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: TIMP-2
Long Name
Tissue Inhibitors of Metalloproteinases 2
Alternate Names
TIMP2
Gene Symbol
TIMP2
UniProt
Additional TIMP-2 Products
Product Documents for TIMP-2 Antibody (TIMP2/2044)
Product Specific Notices for TIMP-2 Antibody (TIMP2/2044)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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