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TIMP-3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21434

Novus Biologicals, part of Bio-Techne
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NBP3-21434-25ul
NBP3-21434-100ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for TIMP-3 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human TIMP-3 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.

Scientific Data Images for TIMP-3 Antibody

TIMP-3 Antibody Immunoprecipitation: TIMP-3 Antibody [NBP3-21434]

Immunoprecipitation: TIMP-3 Antibody [NBP3-21434]

TIMP-3 was immunoprecipitated using NBP3-21434 at 1:100 dilution, 60 ug of Immunomagnetic beads Protein A/G, and secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size :27 kDa
TIMP-3 Antibody

Immunoprecipitation: TIMP-3 Antibody [NBP3-21434] -

Immunoprecipitation: TIMP-3 Antibody [NBP3-21434] - TIMP-3 was immunoprecipitated using: Lane A:0.5 mg MCF-7 Whole Cell Lysate 4 µL NBP3-21434 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-21434,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 24 kDa Observed band size :27 kDa

Applications for TIMP-3 Antibody

Application
Recommended Usage

Immunoprecipitation

1-5 uL/mg of lysate
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS, pH 7.0

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TIMP-3

TIMPs form tight inhibitory complexes with MMPs, which are involved in matrix degradation and in tumor invasiveness and metastasis. TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Long Name

Tissue Inhibitors of Metalloproteinases 3

Alternate Names

TIMP3

Gene Symbol

TIMP3

Additional TIMP-3 Products

Product Documents for TIMP-3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TIMP-3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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