TLR9 Antibody (26C593.2) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24729F

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Equine, Monkey, Primate

Cited:

Human

Applications

Validated:

Block/Neutralize, CyTOF-ready, Dot Blot, ELISA, Flow (Intracellular), Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro assay, Knockdown Validated, Simple Western, Western Blot

Cited:

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 26C593.2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TLR9 Antibodies »

Product Specifications

Immunogen

This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037).

Reactivity Notes

Rhesus Monkey.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TLR9 Antibody (26C593.2) [FITC]

Flow Cytometry: TLR9 Antibody (26C593.2) [FITC] [NBP2-24729F] - Analysis using the FITC conjugate of NBP2-24729. Staining of TLR9 in Ramos cells using 0.5 ug of this antibody. Green represents isotype control; red represents anti-TLR9 antibody. TLR intracellular flow kit was used for this test.
Flow Cytometry: TLR9 Antibody (26C593.2) [FITC] [NBP2-24729F] - Flow cytometric analysis of TLR9 in human B cells using 1ug/10^6 cells of NBP2-24908. Cells were then fixed and permeabilized. Then stained with 1 ug of FITC-conjugated TLR9 antibody or the isotype control.
Flow Cytometry: TLR9 Antibody (26C593.2) [FITC] [NBP2-24729F] - Analysis using the FITC conjugated version of NBP2-24729. Staining of TLR9 in Ramos cells using 0.5 ug of this antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control; purple represents anti-TLR9 antibody.

Applications for TLR9 Antibody (26C593.2) [FITC]

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Dot Blot

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

In vitro assay

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: TLR9

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.

Long Name

Toll-like Receptor 9

Alternate Names

CD289

Gene Symbol

TLR9

Additional TLR9 Products

Product Documents for TLR9 Antibody (26C593.2) [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TLR9 Antibody (26C593.2) [FITC]

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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