TLR9 Antibody (26C593.2) - Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24863

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Equine, Monkey, Primate

Cited:

Human, Primate (Rhesus monkey)

Applications

Validated:

CyTOF-ready, ELISA, Flow (Cell Surface), Flow (Intracellular), Flow Cytometry, Functional, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro assay, Knockdown Validated, Simple Western, Western Blot

Cited:

Flow Cytometry Control, IF/IHC, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vitro assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 26C593.2

Format

Azide Free

Concentration

1.0 mg/ml

View all TLR9 Antibodies »

Product Specifications

Immunogen

This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-300 of human TLR9 isoform A (Genbank accession no. AAF78037).

Reactivity Notes

Rhesus Monkey.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TLR9 Antibody (26C593.2) - Azide Free

Western Blot: TLR9 Antibody (26C593.2)Azide Free [NBP2-24863]

Western Blot: TLR9 Antibody (26C593.2)Azide Free [NBP2-24863]

Western Blot: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Analysis of TLR9 in A) human PBMC, B) human intestine, C) mouse intestine, and D) rat intestine tissue lysates using this antibody at a dilution of 3 ug/ml.
Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]

Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]

Immunohistochemistry-Paraffin: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Formalin-fixed, paraffin-embedded human spleen probed with TLR9 antibody at 5 ug/ml. Human tissue was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]

Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863]

Flow Cytometry: TLR9 Antibody (26C593.2) - Azide Free [NBP2-24863] - Intracellular flow analysis of TLR9 in Ramos cells using 0.5 ug of this antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control; purple represents anti-TLR9 antibody.
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Applications for TLR9 Antibody (26C593.2) - Azide Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow (Intracellular)

1:10 - 1:1000

Flow Cytometry

1ul/1 million cells

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Immunoprecipitation

1:10 - 1:500

Simple Western

30 ug/ml

Western Blot

2-5 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. In human PBMC, a ~120 kDa band is observed. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 17116765). Use in Immunohistochemistry reported in scientific literature (PMID: 16477017). Use in FLOW cell surface reported in scientific literature (PMID: 15879022).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Sterile - filtered PBS

Format

Azide Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TLR9

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.

Long Name

Toll-like Receptor 9

Alternate Names

CD289

Gene Symbol

TLR9

Additional TLR9 Products

Product Documents for TLR9 Antibody (26C593.2) - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TLR9 Antibody (26C593.2) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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