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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Validated:

Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen

Cited:

Flow (Cell Surface), Immunohistochemistry-Frozen

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 12A380

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE was used as immunogen.

Reactivity Notes

Cross reacts with Human and Mouse.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TRANCE/TNFSF11/RANK L Antibody (12A380) [Alexa Fluor® 647]

Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) [Alexa Fluor (R) 647] [NBP2-27390] - Cell surface staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay.

Applications for TRANCE/TNFSF11/RANK L Antibody (12A380) [Alexa Fluor® 647]

Application
Recommended Usage

Flow Cytometry

1ul/1 million cells

Immunohistochemistry-Frozen

1:10-1:500
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TRANCE/TNFSF11/RANK L

TRANCE/OPGL/RANkL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANkL {receptor activator of NF-kB (RANk) ligand} has been implicated in interactions between T cells and DCs. RANkL (RANk Ligand) binds to RANk on DCs, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in DC survival. RANkL is also important in T- and B-cell maturation. As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), RANkL can both activate mature osteoclasts and mediate osteoclastogenesis. RANkL deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.

Long Name

TNF-related Activation-induced Cytokine

Alternate Names

CD254, ODF, OPGL, RANK L, RANKL, TNFSF11

Gene Symbol

TNFSF11

Additional TRANCE/TNFSF11/RANK L Products

Product Documents for TRANCE/TNFSF11/RANK L Antibody (12A380) [Alexa Fluor® 647]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRANCE/TNFSF11/RANK L Antibody (12A380) [Alexa Fluor® 647]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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